Faculty of Medicine, University of Prishtina, 10 000 Prishtina, Kosovo.
Faculty of Natural Sciences and Mathematics, Institute of Biology, Ss. Cyril and Methodius University in Skopje, 1000 Skopje, North Macedonia.
Exp Biol Med (Maywood). 2023 Sep;248(17):1492-1499. doi: 10.1177/15353702231199468. Epub 2023 Oct 14.
Hyperoxia exposure of immature lungs contributes to lung injury and airway hyperreactivity. Up to now, treatments of airway hyperreactivity induced by hyperoxia exposure have been ineffective. The aim of this study was to investigate the effects of quercetin on hyperoxia-induced airway hyperreactivity, impaired relaxation, and lung inflammation. Newborn rats were exposed to hyperoxia (FiO > 95%) or ambient air (AA) for seven days. Subgroups were injected with quercetin (10 mg·kg·day). After exposures, tracheal cylinders were prepared for wire myography. Contraction to methacholine was measured in the presence or absence of organ bath quercetin and/or -nitro-L-arginine methyl ester (L-NAME). Relaxation responses were evoked in preconstricted tissues using electrical field stimulation (EFS). Lung tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) levels were measured by enzyme-linked immunosorbent assay (ELISA). A < 0.05 was considered statistically significant. Contractile responses of tracheal smooth muscle (TSM) of hyperoxic animals were significantly increased compared with AA animals ( < 0.001). Treatment with quercetin significantly reduced contraction in hyperoxic groups compared with hyperoxic control ( < 0.01), but did not have any effect in AA groups. In hyperoxic animals, relaxation of TSM was significantly reduced compared with AA animals ( < 0.001), while supplementation of quercetin restored the lost relaxation in hyperoxic groups. Incubation of preparations in L-NAME significantly reduced the quercetin effects on both contraction and relaxation ( < 0.01). Treatment of hyperoxic animals with quercetin significantly decreased the expression of TNF-α and IL-1β compared with hyperoxic controls ( < 0.001 and < 0.01, respectively).The findings of this study demonstrate the protective effect of quercetin on airway hyperreactivity and suggest that quercetin might serve as a novel therapy to prevent and treat neonatal hyperoxia-induced airway hyperreactivity and inflammation.
高氧暴露会导致未成熟肺损伤和气道高反应性。到目前为止，高氧暴露引起的气道高反应性的治疗一直没有效果。本研究旨在探讨槲皮素对高氧诱导的气道高反应性、松弛受损和肺部炎症的影响。新生大鼠暴露于高氧（FiO > 95%）或环境空气（AA）中 7 天。亚组接受槲皮素（10mg·kg·天）注射。暴露后，准备气管圆柱进行金属丝肌动描记术。在存在或不存在器官浴槲皮素和/或 -硝基-L-精氨酸甲酯（L-NAME）的情况下测量对乙酰甲胆碱的收缩。使用电场刺激（EFS）在预收缩组织中诱发松弛反应。通过酶联免疫吸附试验（ELISA）测量肺肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。 < 0.05 被认为具有统计学意义。与 AA 动物相比，高氧动物的气管平滑肌（TSM）的收缩反应明显增加（ < 0.001）。与高氧对照组相比，槲皮素治疗显著降低了高氧组的收缩（ < 0.01），但对 AA 组没有任何影响。与 AA 动物相比，高氧动物的 TSM 松弛明显减少（ < 0.001），而槲皮素的补充恢复了高氧组丧失的松弛。在 L-NAME 孵育的制剂中，槲皮素对收缩和松弛的作用显著降低（ < 0.01）。与高氧对照组相比，用槲皮素治疗高氧动物显著降低 TNF-α和 IL-1β的表达（ < 0.001 和 < 0.01）。本研究的结果表明槲皮素对气道高反应性具有保护作用，并表明槲皮素可能作为一种新的治疗方法，预防和治疗新生儿高氧诱导的气道高反应性和炎症。
