Anderson W A, Gabriel B W, Nerurkar S G, Wyche J H, Yates P E, Hanker J S
J Submicrosc Cytol. 1986 Oct;18(4):683-90.
In estrogen and diethylstilbestrol-treated rats, uterine peroxidases originate from two sources, the infiltrating eosinophils (exogenous) and the uterine tissue itself (endogenous). The study reported here distinguished the exogenous peroxidases by biochemical means and by cytochemistry. Eosinophil peroxidases are confined to the stromal and myometrial regions and appear simultaneously with endogenous peroxidases. At 48 h after estrogen-administration, the clear uterine luminal washings contain five peroxidase isoforms; this increases to 7-15 isozymes by 72 h. Uterine fluid peroxidase isozymes are acidic proteins with pI values ranging from pH 4.0 - 7.2, while the principal eosinophil peroxidase is a basic protein with a pI value ranging from pH 8.0-8.9. Eosinophil peroxidase is electrophoretically demonstrable only in the presence of the cationic detergent cetyltrimethyl ammonium bromide (CTAB) and has a spectrophotometric optimum of pH 4.4. In contrast, uterine fluid peroxidases have a pH optimum of 7.2. and no requirement for CTAB. Uterine tissue peroxidase extracted in the presence of Ca2+, showed a minor electrophoretic peroxidase band in the acidic pH range; however, a CTAB-activated peroxidase similar to the principal eosinophil peroxidase appeared as a basic protein. The data strongly suggest that uterine fluid peroxidases are estrogen-induced peroxidases (EIP) distinct from the eosinophil peroxidases that are largely restricted to the stromal compartment. This conclusion is supported by cytochemical studies that show two eosinophilperoxidases. The one shown by DAB was resistant to cyanide whereas the one shown by PPD/PC was inhibited by cyanide. A uterine tissue peroxidase, which was demonstrated only in stromal cells by the DAB medium, was more sensitive to cyanide than the eosinophil peroxidase shown by DAB.(ABSTRACT TRUNCATED AT 250 WORDS)
在雌激素和己烯雌酚处理的大鼠中，子宫过氧化物酶有两个来源，即浸润的嗜酸性粒细胞（外源性）和子宫组织本身（内源性）。本文报道的研究通过生化方法和细胞化学区分了外源性过氧化物酶。嗜酸性粒细胞过氧化物酶局限于基质和肌层区域，并与内源性过氧化物酶同时出现。给予雌激素后48小时，清亮的子宫腔冲洗液中含有五种过氧化物酶同工型；到72小时时增加到7 - 15种同工酶。子宫液过氧化物酶同工型是酸性蛋白，其pI值范围为pH 4.0 - 7.2，而主要的嗜酸性粒细胞过氧化物酶是碱性蛋白，pI值范围为pH 8.0 - 8.9。嗜酸性粒细胞过氧化物酶只有在阳离子去污剂十六烷基三甲基溴化铵（CTAB）存在时才能通过电泳显示，其分光光度法最适pH为4.4。相比之下，子宫液过氧化物酶的最适pH为7.2，且不需要CTAB。在Ca2 +存在下提取的子宫组织过氧化物酶在酸性pH范围内显示出一条较小的电泳过氧化物酶带；然而，一种与主要嗜酸性粒细胞过氧化物酶相似的CTAB激活过氧化物酶表现为碱性蛋白。数据强烈表明，子宫液过氧化物酶是雌激素诱导的过氧化物酶（EIP），与主要局限于基质区室的嗜酸性粒细胞过氧化物酶不同。这一结论得到了细胞化学研究的支持，该研究显示有两种嗜酸性粒细胞过氧化物酶。DAB显示的一种对氰化物有抗性，而PPD/PC显示的一种被氰化物抑制。一种仅在基质细胞中由DAB培养基显示的子宫组织过氧化物酶比DAB显示的嗜酸性粒细胞过氧化物酶对氰化物更敏感。（摘要截断于250字）
