Morikawa S, Kikuchi Y, Sekiya S, Takamizawa H
Acta Obstet Gynaecol Jpn. 1981 Apr;33(4):499-505.
Uterine Peroxidase (UP) can be induced by the administration of estradiol (E2) in the rat. We studied whether this biochemical parameter can be a specific marker of estrogen-dependence of rat uterus. 1. UP content in both immature castrated mature rat uteri was negligible. Whereas UP could be detected after vaginal openning of intact rats. 2. UP induction by E2 administration was first detected at 15-day-old rat uteri. 3. UP induction by E2 or mare serum gonadotropin was detected in both immature and castrated mature rats with the increase of uterine weight. 4. UP induction by E2 was observed only in estrogen-dependent tissues, such as uterus and vagina. 5. UP induction by E2 was prominently suppressed by the treatment with actinomycin-D, cycloheximide and tamoxifen. These results suggest that UP induction by E2 is an useful specific marker when hormone-dependence of rat uterus is investigated.
子宫过氧化物酶（UP）可通过给大鼠注射雌二醇（E2）诱导产生。我们研究了这一生化参数是否可作为大鼠子宫雌激素依赖性的特异性标志物。1. 未成熟去势成熟大鼠子宫中的UP含量可忽略不计。而完整大鼠阴道开口后可检测到UP。2. 在15日龄大鼠子宫中首次检测到E2诱导的UP。3. 在未成熟和去势成熟大鼠中，随着子宫重量增加，E2或马血清促性腺激素诱导的UP均可被检测到。4. 仅在雌激素依赖性组织，如子宫和阴道中观察到E2诱导的UP。5. 放线菌素-D、环己酰亚胺和他莫昔芬处理可显著抑制E2诱导的UP。这些结果表明，在研究大鼠子宫的激素依赖性时，E2诱导的UP是一个有用的特异性标志物。
