Department of Oral Pathology and Medicine, Obafemi Awolowo University Teaching Hospital Complexes, Ile- Ife, Osun State, Nigeria.
Department of Oral Diagnostic Sciences, Aminu Kano Teaching Hospital/ Bayero University, Kano, Kano, Nigeria.
J Stomatol Oral Maxillofac Surg. 2023 Dec;124(6 Suppl 2):101654. doi: 10.1016/j.jormas.2023.101654. Epub 2023 Oct 12.
Recurrent Aphthous Stomatitis (RAS) is a frequent chronic disease of the oral cavity that affects 5-25 % of the population with a plethora of predisposing factors. Despite its equivocal etiology, immune alterations, hematologic deficiencies and oxidative stress has been reported to be significant etiologic factors. Stress and obesity are other environmental factors that have been studied to understand associations with RAS.
This study investigated the association of stress, hematologic parameters, oxidative indices and other selected salivary factors in a case control study on RAS Methods: Twenty-two participants each in both case and control groups were recruited with saliva and serum samples collected from them after a self-administered Recent Life Changing Questionnaire (RLCQ). OHI-S and salivary flow rate (SFR) were calculated with selected hematologic parameters and oxidative indices such as Total oxidant (TOS) and anti-oxidant (TAS) levels and their ratio - Oxidative Stress Index (OSI). Anti-oxidative indices of Ferric-Reducing Antioxidant Power (FRAP) and Glutathione Peroxidase Activity (GsPHx) were also estimated.
The RAS group had a significantly higher RLCQ scores at a median of 145 more than the control (57.5). There was no significant in their obesity indices, however there was a significant higher mean in the ESR (p< 0.0001) and Vit B12 (p = 0.0001); OHI-S was also significantly higher in the RAS group with a median of 1.65. Both the salivary and serum TOS were significantly higher in the RAS (10.0 ± 3.8, 15.4 ± 8.9) compared to the control group (7.92 ± 1.49, 9.56 ± 3.5). GsPHx activity was significantly higher in both the saliva and serum in the control group (0.08 ± 0.08, 0.19 ± 0.11) while nil significant difference was found in the FRAP activity. Regression showed most important variables to be the salivary GsPHx activity, followed by serum OSI and GsPHx activity.
The oxidative indices of TOS, TAS and GsPHx can serve as significant biomarkers in detecting RAS. This further corroborates the role of immune dysregulation in the etiology and predisposition to RAS.
复发性阿弗他口炎（RAS）是一种常见的口腔慢性疾病，影响 5-25%的人群，存在多种诱发因素。尽管其病因尚不明确，但免疫改变、血液学缺陷和氧化应激已被报道为重要的病因因素。压力和肥胖是其他已被研究以了解与 RAS 相关的环境因素。
本研究通过病例对照研究调查了 RAS 患者应激、血液学参数、氧化指标和其他选定唾液因素之间的关系。
在这项病例对照研究中，每组 22 名参与者，分别从他们那里采集唾液和血清样本，之后让他们自行填写近期生活变化问卷（RLCQ）。通过计算总氧化应激（TOS）和抗氧化（TAS）水平及其比值-氧化应激指数（OSI）等选定的血液学参数和氧化指标，以及唾液铁还原抗氧化能力（FRAP）和谷胱甘肽过氧化物酶活性（GsPHx）的抗氧化指标，来计算 OHI-S 和唾液流量率（SFR）。
RAS 组的 RLCQ 评分中位数为 145，明显高于对照组（57.5）。两组肥胖指数无显著差异，但 ESR（p<0.0001）和 Vit B12（p=0.0001）均值显著较高；RAS 组的 OHI-S 也明显较高，中位数为 1.65。与对照组相比，RAS 组的唾液和血清 TOS 均显著升高（分别为 10.0±3.8、15.4±8.9）。对照组唾液和血清 GsPHx 活性均显著升高（分别为 0.08±0.08、0.19±0.11），而 FRAP 活性无显著差异。回归分析显示，最重要的变量是唾液 GsPHx 活性，其次是血清 OSI 和 GsPHx 活性。
TOS、TAS 和 GsPHx 的氧化指标可作为检测 RAS 的重要生物标志物。这进一步证实了免疫失调在 RAS 的病因和易感性中的作用。
