Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China.
Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China.
Carbohydr Polym. 2023 Dec 15;322:121337. doi: 10.1016/j.carbpol.2023.121337. Epub 2023 Aug 30.
Efficient and stable catalysis has always been the core concept of enzyme catalysis in industrial processes for manufacturing. Here, we constructed molecular enrichment accelerators to synergistically enhance enzyme activity and stability by assembling enzyme surface grafted polymer and cyclodextrin. At 40 °C, the enzyme activity of CalB-PNIPAM/β-CD was 2.9 times that of CalB-PNIPAM. The enzyme activity of CalB-PNIPAM/γ-CD had reached 1.61 times that of CalB. At the same time, the stability of CalB-PNIPAM/β-CD and CalB-PNIPAM/γ-CD are slightly better than that of CalB under high temperature, organic solution and extreme pH conditions. The synergistic increase in activity and stability of the lipase-polymer assembly was achieved due to the structure of assembly, in which the role of cyclodextrin could enrich substrate affecting molecular diffusion. In addition, the lipase-polymer assembly proved to be an efficient catalyst for biodiesel synthesis, with a biodiesel conversion 1.4 times that of CalB at 60 °C. Therefore, this simple and low-cost lipase-polymer assembly provides new possibilities for the construction of high-efficiency industrial biocatalytic catalysts.
高效稳定的催化一直是工业制造过程中酶催化的核心概念。在这里，我们构建了分子富集加速剂，通过组装酶表面接枝聚合物和环糊精来协同增强酶的活性和稳定性。在 40°C 时，CalB-PNIPAM/β-CD 的酶活性是 CalB-PNIPAM 的 2.9 倍。CalB-PNIPAM/γ-CD 的酶活性达到了 CalB 的 1.61 倍。同时，在高温、有机溶剂和极端 pH 条件下，CalB-PNIPAM/β-CD 和 CalB-PNIPAM/γ-CD 的稳定性略优于 CalB。由于组装的结构，脂肪酶-聚合物组装的活性和稳定性协同增加，其中环糊精的作用可以富集影响分子扩散的底物。此外，脂肪酶-聚合物组装被证明是一种高效的生物柴油合成催化剂，在 60°C 时，生物柴油转化率是 CalB 的 1.4 倍。因此，这种简单且低成本的脂肪酶-聚合物组装为构建高效工业生物催化催化剂提供了新的可能性。
