Gupta Taruna, Singhal Tripti, Dhir Sunny, Chandel Vanita, Rishi Narayan, Raj S K, Srivastava Ashish
Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, 201313 India.
Central Research Cell, Department of Biosciences and Technology, Maharishi Markandeshwar, Mullana, Ambala, 133207 India.
3 Biotech. 2023 Nov;13(11):360. doi: 10.1007/s13205-023-03778-7. Epub 2023 Oct 11.
In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and . Agroinfiltration induced yellow mosaic symptoms in mungbean and plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.
The online version contains supplementary material available at 10.1007/s13205-023-03778-7.
在本研究中，克隆并测序了来自大豆分离株的绿豆黄花叶印度病毒（MYMIV）DNA-A（MW590720和MW600934）和DNA-B（MW659819和MW659820）的全长组件。对MYMIV两个组件的核苷酸序列分析显示，与东南亚豆类中的MYMIV分离株具有>96%的同一性和密切的亲缘关系。此外，在pCAMBIA1302载体中构建了MYMIV的二聚体感染性克隆，并建立了针对绿豆、大豆和[此处原文缺失一种植物名称]的种子浸润方案。农杆菌浸润在浸润3周后在绿豆和[此处原文缺失一种植物名称]植株中诱导出黄花叶症状，通过使用MYMIV特异性DNA引物的PCR进一步证实。
在线版本包含可在10.1007/s13205-023-03778-7获取的补充材料。
