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利用反义RNA构建对印度绿豆黄花叶病毒的抗性

Engineering Resistance Against Mungbean yellow mosaic India virus Using Antisense RNA.

作者信息

Haq Q M I, Ali Arif, Malathi V G

机构信息

Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.

出版信息

Indian J Virol. 2010 Jun;21(1):82-5. doi: 10.1007/s13337-010-0003-2. Epub 2010 Sep 3.

DOI:10.1007/s13337-010-0003-2
PMID:23637483
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3550775/
Abstract

Yellow mosaic disease of cultivated legumes in South-East Asia, is caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus of the family Geminiviridae. Efforts to engineer resistance against the genus Begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. Here we have targeted a complementary-sense gene (ACI) encoding Replication initiation Protein (Rep) to develop resistance against soybean isolate of Mungbean yellow mosaic India virus-[India:New Delhi:Soybean 2:1999], a bipartite begomovirus prevalent throughout the Indian subcontinent. We show that the legume host plants co-agroinoculated with infectious constructs of soybean isolate of Mungbean yellow mosaic India virus [India:New Delhi:Soybean 2:1999] along with this antisense Rep gene construct show resistance to the virus.

摘要

东南亚栽培豆类的黄花叶病，是由属于双生病毒科菜豆金色花叶病毒属的印度绿豆黄花叶病毒（MYMIV）和绿豆黄花叶病毒（MYMV）引起的。针对菜豆金色花叶病毒属构建抗性的工作主要集中在使参与病毒复制的互补链病毒基因沉默。在此，我们靶向一个编码复制起始蛋白（Rep）的互补链基因（ACI），以培育对印度绿豆黄花叶病毒- [印度：新德里：大豆2：1999]大豆分离株的抗性，这是一种在整个印度次大陆普遍存在的双分体菜豆金色花叶病毒。我们发现，与印度绿豆黄花叶病毒[印度：新德里：大豆2：1999]大豆分离株的感染性构建体以及该反义Rep基因构建体共同农杆菌接种的豆科寄主植物对该病毒表现出抗性。

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Engineering Resistance Against Mungbean yellow mosaic India virus Using Antisense RNA.利用反义RNA构建对印度绿豆黄花叶病毒的抗性
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本文引用的文献

1
Geminivirus strain demarcation and nomenclature.双生病毒株系划分与命名
Arch Virol. 2008;153(4):783-821. doi: 10.1007/s00705-008-0037-6. Epub 2008 Feb 7.
2
Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco.来自绿豆黄花叶病毒-豇豆的全长和截短Rep基因的表达抑制转基因烟草中的病毒复制。
Virus Genes. 2006 Dec;33(3):365-74. doi: 10.1007/s11262-006-0077-5.
3
Broad spectrum resistance to ssDNA viruses associated with transgene-induced gene silencing in cassava.木薯中与转基因诱导的基因沉默相关的对单链DNA病毒的广谱抗性。
Plant Mol Biol. 2004 Nov;56(4):601-11. doi: 10.1007/s11103-004-0147-9.
4
Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated.番茄黄化曲叶撒丁岛病毒的复制相关蛋白（Rep）介导的对同源和异源双生病毒的抗性通过不同机制产生，并且如果病毒介导的转基因沉默被激活，这种抗性就会被克服。
J Virol. 2003 Jun;77(12):6785-98. doi: 10.1128/jvi.77.12.6785-6798.2003.
5
RNAi targeting of DNA virus in plants.植物中针对DNA病毒的RNA干扰
Nat Biotechnol. 2003 Feb;21(2):131-2. doi: 10.1038/nbt0203-131b.
6
Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation.双生病毒：植物DNA复制、转录及细胞周期调控的模型
Crit Rev Biochem Mol Biol. 2000;35(2):105-40.
7
Functional domains of a geminivirus replication protein.双生病毒复制蛋白的功能结构域。
J Biol Chem. 1997 Apr 11;272(15):9840-6. doi: 10.1074/jbc.272.15.9840.
8
Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA.利用反义RNA构建对番茄黄化曲叶病毒（TYLCV）的抗性
Plant Mol Biol. 1997 Jan;33(2):351-7. doi: 10.1023/a:1005715805000.
9
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51. doi: 10.1073/pnas.77.12.7347.
10
Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus.在转基因烟草中反义病毒基因的表达赋予了对DNA病毒番茄金色花叶病毒的抗性。
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6721-5. doi: 10.1073/pnas.88.15.6721.