利用反义RNA构建对印度绿豆黄花叶病毒的抗性
Engineering Resistance Against Mungbean yellow mosaic India virus Using Antisense RNA.
作者信息
Haq Q M I, Ali Arif, Malathi V G
机构信息
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.
出版信息
Indian J Virol. 2010 Jun;21(1):82-5. doi: 10.1007/s13337-010-0003-2. Epub 2010 Sep 3.
Abstract
Yellow mosaic disease of cultivated legumes in South-East Asia, is caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus of the family Geminiviridae. Efforts to engineer resistance against the genus Begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. Here we have targeted a complementary-sense gene (ACI) encoding Replication initiation Protein (Rep) to develop resistance against soybean isolate of Mungbean yellow mosaic India virus-[India:New Delhi:Soybean 2:1999], a bipartite begomovirus prevalent throughout the Indian subcontinent. We show that the legume host plants co-agroinoculated with infectious constructs of soybean isolate of Mungbean yellow mosaic India virus [India:New Delhi:Soybean 2:1999] along with this antisense Rep gene construct show resistance to the virus.
摘要
东南亚栽培豆类的黄花叶病，是由属于双生病毒科菜豆金色花叶病毒属的印度绿豆黄花叶病毒（MYMIV）和绿豆黄花叶病毒（MYMV）引起的。针对菜豆金色花叶病毒属构建抗性的工作主要集中在使参与病毒复制的互补链病毒基因沉默。在此，我们靶向一个编码复制起始蛋白（Rep）的互补链基因（ACI），以培育对印度绿豆黄花叶病毒- [印度：新德里：大豆2：1999]大豆分离株的抗性，这是一种在整个印度次大陆普遍存在的双分体菜豆金色花叶病毒。我们发现，与印度绿豆黄花叶病毒[印度：新德里：大豆2：1999]大豆分离株的感染性构建体以及该反义Rep基因构建体共同农杆菌接种的豆科寄主植物对该病毒表现出抗性。
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