Department of Neurology, Hangzhou Third People's Hospital, Hangzhou, Zhejiang, China.
Department of Neurology, Hangzhou First People's Hospital, Hangzhou, Zhejiang, China.
Bioengineered. 2021 Dec;12(1):2851-2862. doi: 10.1080/21655979.2021.1940073.
Cerebral ischemic stroke (CIS) is the most common type of stroke, which is highly hazardous. This investigation aims to analyze the correlation of miR-497 with CIS, so as to provide reliable evidence for clinical response to CIS and lay a solid foundation for follow-up research. Eighty-nine CIS patients and 39 concurrent physical examinees selected between June 2017 and October 2018 were enrolled as the research participants. Additionally, SD rats with increased miR-497 expression and normal SD rats were purchased for CIS modeling to observe the clinical implications of miR-497 in CIS, as well as the water content of brain tissue and neuronal apoptosis of rats. miR-497 expression was lower in CIS patients than in physical examinees, and that in patients with complete stroke (CS) was the lowest, which increased after treatment. As determined by the receiver operating characteristic curve (ROC) analysis, miR-497 had an outstanding diagnostic efficacy for CIS and was negatively correlated with the National Institutes of Health Stroke Scale (NIHSS) and MDA concentration, while positively related to SOD concentration. Prognostic follow-up demonstrated that decreased miR-497 expression in patients after treatment predicted an increased risk of prognostic death and recurrence. However, observed in rats, the water content of the brain tissue of rats with increased miR-497 expression was reduced, and the neuronal apoptosis rate of the brain tissue was inhibited. Taken together, with low expression in CIS, miR-497 is strongly related to CIS progression and is a candidate CIS marker.
脑缺血性脑卒中（CIS）是最常见的脑卒中类型，具有很高的危险性。本研究旨在分析 miR-497 与 CIS 的相关性，为临床治疗 CIS 提供可靠的依据，并为后续研究奠定坚实的基础。将 2017 年 6 月至 2018 年 10 月期间收治的 89 例 CIS 患者和 39 例同期体检者纳入研究对象。此外，购买 miR-497 表达增加的 SD 大鼠和正常 SD 大鼠进行 CIS 造模，观察 miR-497 在 CIS 中的临床意义，以及大鼠脑组织含水量和神经元凋亡情况。CIS 患者 miR-497 表达低于体检者，完全性脑卒中（CS）患者最低，治疗后升高。通过受试者工作特征曲线（ROC）分析，miR-497 对 CIS 具有出色的诊断效果，与 NIHSS 和 MDA 浓度呈负相关，与 SOD 浓度呈正相关。预后随访表明，治疗后患者 miR-497 表达降低预示着预后死亡和复发的风险增加。然而，在大鼠中观察到，miR-497 表达增加的大鼠脑组织含水量降低，脑组织神经元凋亡率受到抑制。综上所述，miR-497 在 CIS 中表达较低，与 CIS 进展密切相关，是 CIS 的候选标志物。
