Benton Lauren, Lopez-Galvez Nicolas, Herman Chloe, Caporaso Greg, Cope Emily, Rosales Cecilia, Gameros Mercedes, Lothrop Nathan, Martínez Fernando, Wright Anne, Carr Tara, Beamer Paloma
University of Arizona.
Northern Arizona University.
Res Sq. 2023 Sep 29:rs.3.rs-3325336. doi: 10.21203/rs.3.rs-3325336/v1.
We previously reported that asthma prevalence was higher in the United States (US) compared to Mexico (MX) (25.8% vs 8.4%). This investigation assessed differences in microbial dust composition in relation to demographic and housing characteristics on both sides of the US-MX Border. Forty homes were recruited in the US and MX. Home visits collected floor dust and documented occupants' demographics, asthma prevalence, and housing structure and use characteristics. US households were more likely to have inhabitants who reported asthma when compared with MX households (30% vs 5%) and had significantly different flooring types. The percentage of households on paved roads, with flushing toilets, with piped water and with air conditioning was higher in the US, while dust load was higher in MX. Significant differences exist between countries in the microbial composition of the floor dust. Dust from US homes was enriched with , whereas dust from Mexican homes was enriched with and . A predictive metagenomics analysis identified 68 significantly differentially abundant functional pathways between US and MX. This study documented multiple structural, environmental, and demographic differences between homes in the US and MX that may contribute to significantly different microbial composition of dust observed in these two countries.
我们之前报道过，与墨西哥（MX）相比，美国（US）的哮喘患病率更高（25.8% 对 8.4%）。本调查评估了美墨边境两侧与人口统计学和住房特征相关的微生物灰尘成分差异。在美国和墨西哥招募了40户家庭。家访收集了地板灰尘，并记录了居住者的人口统计学信息、哮喘患病率以及住房结构和使用特征。与墨西哥家庭相比，美国家庭中报告患有哮喘的居民更有可能（30% 对 5%），并且地板类型有显著差异。美国铺设道路、有冲水马桶、有自来水和有空调的家庭比例更高，而墨西哥的灰尘负荷更高。两国地板灰尘的微生物组成存在显著差异。美国家庭的灰尘富含 ，而墨西哥家庭的灰尘富含 和 。一项预测宏基因组学分析确定了美国和墨西哥之间68条显著不同的功能丰富通路。这项研究记录了美国和墨西哥家庭之间在结构、环境和人口统计学方面的多种差异，这些差异可能导致这两个国家观察到的灰尘微生物组成显著不同。
