Ni Yang, Li Jingling, Lu Qianqi, Chen Haimei
Center for Bioinformatics, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR China.
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, PR China.
Mitochondrial DNA B Resour. 2023 Oct 11;8(10):1071-1076. doi: 10.1080/23802359.2023.2265100. eCollection 2023.
With its nearly 200 species, the genus is the most species-rich within the Cactaceae family, yet surprisingly, few of its chloroplast genomes have been studied. We focused on the species DC. 1828, a petite cactus native to Mexico and favored by horticulturists, yet whose phylogenetic relationships remain uncertain due to a lack of genomic data. We extracted the DNA from a sample obtained in China, sequenced it using the NovaSeq 6000 platform, and assembled the chloroplast genome using GetOrganelle software. Our assembly resulted in a chloroplast genome of 110,981 base pairs with an overall GC content of 36.28%, which included 100 genes (95 unique). Notably, several protein-coding genes were absent. Phylogenetic analysis using 59 shared genes across nine species and one Obregonia species revealed that and are closely related, suggesting a recent common ancestor and possible shared evolutionary pressures or ecological niches. This study provides crucial genomic data for and hints at intriguing phylogenetic relationships within the genus.
该属有近200个物种，是仙人掌科中物种最为丰富的属，但令人惊讶的是，对其叶绿体基因组的研究却很少。我们重点研究了1828年命名的一种物种，它是一种原产于墨西哥的小型仙人掌，深受园艺学家喜爱，但由于缺乏基因组数据，其系统发育关系仍不明确。我们从在中国获取的一个样本中提取了DNA，使用NovaSeq 6000平台对其进行测序，并使用GetOrganelle软件组装叶绿体基因组。我们组装得到了一个110,981个碱基对的叶绿体基因组，总体GC含量为36.28%，其中包含100个基因（95个独特基因）。值得注意的是，有几个蛋白质编码基因缺失。使用九个物种和一个奥布雷贡属物种中的59个共享基因进行的系统发育分析表明，[具体物种名称1]和[具体物种名称2]关系密切，这表明它们有最近的共同祖先以及可能共同面临的进化压力或生态位。这项研究为[具体物种名称1]和[具体物种名称2]提供了关键的基因组数据，并暗示了该属内有趣的系统发育关系。
