Department of Chemistry, Research Center for Natural Resources, Health and the Environment (RENSMA), Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, Fuerzas Armadas Ave., 21007 Huelva, Spain.
Department of Chemistry, Faculty of Sciences, National University of San Antonio Abad of Cusco, Av. de La Cultura, 773 Cusco, Peru.
J Proteome Res. 2023 Nov 3;22(11):3499-3507. doi: 10.1021/acs.jproteome.3c00356. Epub 2023 Oct 16.
Lung cancer (LC) is a leading cause of mortality, claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in its early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to assess the response to treatment or the detection of cancer recurrence. In this study, we have evaluated the metabolomic profile of serum samples ( = 110) from preoperative (PRE) and postoperative (POST) LC patients collected at two different time points (1 month, A; 3-6 months, B) with respect to healthy people. An untargeted metabolomic platform based on reversed phase (RP) and hydrophilic interaction chromatography (HILIC), using ultra-high performance liquid chromatography (UHPLC) and mass spectrometry (MS), was applied (MassIVE ID MSV000092213). Twenty-two altered metabolites were annotated by comparing all the different studied groups. DG(14,0/22:1), stearamide, proline, and E,e-carotene-3,3'-dione were found altered in PRE, and their levels returned to those of a baseline control group 3-6 months after surgery. Furthermore, 3-galactosyllactose levels remained altered after intervention in some patients. This study provides unique insights into the metabolic profiles of LC patients after surgery at two different time points by combining complementary analytical methods.
肺癌（LC）是导致死亡的主要原因，全球每年有超过 180 万人因此死亡。当疾病处于早期阶段时，手术是最有效的治疗方法之一。研究具有治愈意图的手术干预后的代谢改变，可用于评估治疗反应或癌症复发的检测。在这项研究中，我们评估了术前（PRE）和术后（POST）LC 患者血清样本的代谢组学特征（= 110），这些患者在两个不同的时间点（1 个月，A；3-6 个月，B）采集，并与健康人进行了比较。我们应用了基于反相（RP）和亲水相互作用色谱（HILIC）的非靶向代谢组学平台，使用超高效液相色谱（UHPLC）和质谱（MS）（MassIVE ID MSV000092213）。通过比较所有不同的研究组，注释了 22 种改变的代谢物。DG(14,0/22:1)、硬脂酰胺、脯氨酸和 E，e-胡萝卜素-3,3'-二酮在 PRE 时发生改变，并且在手术后 3-6 个月其水平恢复到基线对照组的水平。此外，在一些患者中，3-半乳糖基乳糖的水平在干预后仍然发生改变。本研究通过结合互补的分析方法，提供了关于手术治疗后 LC 患者在两个不同时间点代谢谱的独特见解。
