Nawaz Muhammad Zohaib, Alghamdi Huda Ahmed, Zahoor Mehvish, Rashid Fizzah, Alshahrani Atheer Ahmed, Alghamdi Nuha S, Pugazhendhi Arivalagan, Zhu Daochen
Biofuels Institute, School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
Department of Biology, College of Sciences, King Khalid University, Abha, 61413, Saudi Arabia.
Environ Res. 2024 Jan 15;241:117415. doi: 10.1016/j.envres.2023.117415. Epub 2023 Oct 14.
Periodontitis is a severe form of gum disease caused by bacterial plaque that affects millions of people and has substantial worldwide health and economic implications. However, current clinical antiseptic and antimicrobial drug therapies are insufficient because they frequently have numerous side effects and contribute to widespread bacterial resistance. Recently, nanotechnology has shown promise in the synthesis of novel periodontal therapeutic materials. Nanoparticles are quickly replacing antibiotics in the treatment of bacterial infections, and their potential application in dentistry is immense. The alarming increases in antimicrobial resistance further emphasize the importance of exploring and utilizing nanotechnology in the fight against tooth diseases particularly periodontitis. We developed 16 different combinations of mesoporous silica nanomaterials in this study by ageing, drying, and calcining them with 11 different metals including silver, zinc, copper, gold, palladium, ruthenium, platinum, nickel, cerium, aluminium, and zirconium. The antibacterial properties of metal-doped silica were evaluated using four distinct susceptibility tests. The agar well diffusion antibacterial activity test, which measured the susceptibility of the microbes being tested, as well as the antibacterial efficacy of mesoporous silica with different silica/metal ratios, were among these studies. The growth kinetics experiment was used to investigate the efficacy of various metal-doped silica nanoparticles on microbial growth. To detect growth inhibitory effects, the colony-forming unit assay was used. Finally, MIC and MBC tests were performed to observe the inhibition of microbial biofilm formation. Our findings show that silver- and zinc-doped silica nanoparticles synthesized using the sol-gel method can be effective antimicrobial agents against periodontitis-causing microbes. This study represents the pioneering work reporting the antimicrobial properties of metal-loaded TUD-1 mesoporous silica, which could be useful in the fight against other infectious diseases too.
牙周炎是一种由细菌菌斑引起的严重牙龈疾病，影响着数百万人，在全球范围内具有重大的健康和经济影响。然而，目前的临床抗菌和抗菌药物治疗并不充分，因为它们经常有许多副作用，并导致广泛的细菌耐药性。最近，纳米技术在新型牙周治疗材料的合成方面显示出了前景。纳米颗粒正在迅速取代抗生素用于治疗细菌感染，其在牙科的潜在应用是巨大的。抗菌耐药性的惊人增加进一步强调了在对抗牙齿疾病特别是牙周炎方面探索和利用纳米技术的重要性。在本研究中，我们通过用包括银、锌、铜、金、钯、钌、铂、镍、铈、铝和锆在内的11种不同金属对介孔二氧化硅纳米材料进行老化、干燥和煅烧，开发了16种不同的组合。使用四种不同的药敏试验评估了金属掺杂二氧化硅的抗菌性能。这些研究包括琼脂孔扩散抗菌活性试验，该试验测量了受试微生物的敏感性，以及不同二氧化硅/金属比例的介孔二氧化硅的抗菌效果。生长动力学实验用于研究各种金属掺杂二氧化硅纳米颗粒对微生物生长的功效。为了检测生长抑制作用，使用了菌落形成单位测定法。最后，进行了MIC和MBC试验以观察对微生物生物膜形成的抑制作用。我们的研究结果表明，使用溶胶-凝胶法合成的银和锌掺杂二氧化硅纳米颗粒可以成为对抗引起牙周炎的微生物的有效抗菌剂。这项研究代表了首次报道负载金属的TUD-1介孔二氧化硅抗菌性能的开创性工作，这在对抗其他传染病方面也可能有用。
