白腐真菌液体培养中全氟辛酸（PFOA）的去除。

Removal of perfluorooctanoic acid (PFOA) in the liquid culture of Phanerochaete chrysosporium.

机构信息

Xi'an International University, Xi'an, Shaanxi 710077, PR China.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China.

出版信息

Chemosphere. 2023 Dec;345:140427. doi: 10.1016/j.chemosphere.2023.140427. Epub 2023 Oct 14.

DOI:10.1016/j.chemosphere.2023.140427

PMID:37844703

Abstract

Perfluorooctanoic acid (PFOA) is becoming a concern due to its persistence, bioaccumulation, and potential harmful effects on humans and the environment. In this study, the fungus Phanerochaete chrysosporium (P. chrysosporium) was used to remove the PFOA in liquid culture system. The results showed that the average activities of laccase (Lac), lignin peroxidase (LiP), and manganese peroxidase (MnP) enzymes secreted by P. chrysosporium were 0.0003 U/mL, 0.013 U/mL, and 0.0059 U/mL, respectively, during the incubation times of 0-75 days. The pH of 3 and incubation time of 45-55 days were the optimum parameters for the three enzymes activities. The enzyme activities in P. chrysosporium incubation system were firstly inhibited by adding PFOA and then they were enhanced after 14 days. The maximum removal efficiency of PFOA (69.23%) was achieved after 35 days in P. chrysosporium incubation system with an initial PFOA concentration of 0.002 mM and no veratryl alcohol (VA). Adsorption was not a main pathway for PFOA removal and the PFOA adsorbed in fungi mycelial mat accounted for merely 1.91%. The possible products of PFOA contained partially fluorinated aldehyde, alcohol, and aromatic ring. These partially fluorinated compounds might result from PFOA degradation via a combination of cross-coupling and rearrangement of free radicals.

摘要

全氟辛酸（PFOA）因其持久性、生物累积性以及对人类和环境的潜在有害影响而受到关注。在本研究中，使用真菌糙皮侧耳（Phanerochaete chrysosporium，P. chrysosporium）在液体培养系统中去除 PFOA。结果表明，在 0-75 天的培养时间内，P. chrysosporium 分泌的漆酶（Lac）、木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）酶的平均活性分别为 0.0003 U/mL、0.013 U/mL 和 0.0059 U/mL。pH 值为 3 和培养时间为 45-55 天是三种酶活性的最佳参数。在添加 PFOA 后，P. chrysosporium 培养系统中的酶活性首先受到抑制，然后在 14 天后增强。在 P. chrysosporium 培养系统中，当初始 PFOA 浓度为 0.002 mM 且没有藜芦醇（VA）时，经过 35 天的反应，PFOA 的去除效率最高（69.23%）。吸附不是 PFOA 去除的主要途径，真菌菌丝体垫中吸附的 PFOA 仅占 1.91%。PFOA 的可能产物包含部分氟化醛、醇和芳香环。这些部分氟化化合物可能是由于 PFOA 通过自由基的交叉偶联和重排降解产生的。