Key Laboratory of Animal Resistance Biology of Shandong Province, Key Laboratory of Food Nutrition and Safety, College of Life Science, Shandong Normal University, Jinan 250014, China; Dongying Institute, Shandong Normal University, Dongying 257000, China.
Key Laboratory of Animal Resistance Biology of Shandong Province, Key Laboratory of Food Nutrition and Safety, College of Life Science, Shandong Normal University, Jinan 250014, China.
J Colloid Interface Sci. 2024 Jan;653(Pt B):1805-1816. doi: 10.1016/j.jcis.2023.10.055. Epub 2023 Oct 13.
BPA aptamers functionalized cerium oxide nanoparticles encapsulated in zeolitic imidazolate framework-8 (CeO@ZIF-8/Apt) were developed to fabricate an all-in-one portable platform for on-site quantitative detection of BPA. By combining biocomposites with a 3,3',5,5'-tetramethylbenzidine (TMB)-based sodium alginate (SA) hydrogel and smartphone-based RGB analysis, highly sensitive and convenient monitoring of BPA was achieved. CeO@ZIF-8 composites were constructed using a novel surfactant-modified concentration-controlled synthesis strategy. After being functionalized with BPA aptamers, CeO@ZIF-8/Apt biocomposites were used as target-response colorimetric probes for target recognition and signal transduction. The oxidase-like activity of CeO@ZIF-8 was effectively sealed by BPA aptamers and controllably released in a concentration-dependent manner through aptamer-BPA reactions. Utilizing SA hydrogels containing TMB in the caps, a one-step sample addition and one-pot detection can be conveniently achieved and reliably quantified by smartphone-based RGB analysis in an instrument-free way. The detection range of this portable detection platform is 50 pg/mL to 500 ng/mL with limit of detection calculated as 34.88 pg/mL, comparable to that of conventional detection in the solution system (4.57 pg/mL). The recoveries in tap water, apple juice, and milk ranged from 91.02 % and 106.75 %. This work contributes new insights into the design of all-in-one detection platforms for contaminants monitoring in resource-constrained regions.
基于铈氧化物纳米粒子封装在沸石咪唑酯骨架-8（CeO@ZIF-8/Apt）中的 BPA 适体被开发用于构建一种用于现场定量检测 BPA 的一体式便携式平台。通过将生物复合材料与基于 3,3',5,5'-四甲基联苯胺（TMB）的海藻酸钠（SA）水凝胶和基于智能手机的 RGB 分析相结合，实现了对 BPA 的高灵敏度和便捷监测。CeO@ZIF-8 复合材料是通过一种新颖的表面活性剂修饰的浓度控制合成策略构建的。在被 BPA 适体功能化后，CeO@ZIF-8/Apt 生物复合材料被用作目标识别和信号转导的目标响应比色探针。CeO@ZIF-8 的氧化酶样活性通过 BPA 适体有效地被密封，并通过适体-BPA 反应以浓度依赖的方式可控释放。利用含有 TMB 的 SA 水凝胶作为帽子，通过智能手机的 RGB 分析可以方便地进行一步样品添加和一锅检测，并且可以在无需仪器的情况下进行可靠的定量分析。该便携式检测平台的检测范围为 50 pg/mL 至 500 ng/mL，检测限计算为 34.88 pg/mL，与传统溶液体系中的检测相当（4.57 pg/mL）。在自来水中、苹果汁和牛奶中的回收率范围为 91.02%和 106.75%。这项工作为在资源有限的地区设计用于污染物监测的一体式检测平台提供了新的思路。
