基于未修饰适配体和阳离子聚合物聚集金纳米粒子的比色法检测双酚A
Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles.
作者信息
Zhang Dongwei, Yang Jiayi, Ye Jing, Xu Lurong, Xu Hanchu, Zhan Shenshan, Xia Bing, Wang Lumei
机构信息
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
School of Agriculture and Biology and Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China; Department of Management, Shanghai Sanda College, Shanghai 201209, China.
出版信息
Anal Biochem. 2016 Apr 15;499:51-56. doi: 10.1016/j.ab.2016.01.011. Epub 2016 Jan 25.
In this study, a colorimetric method was exploited to detect bisphenol A (BPA) based on BPA-specific aptamer and cationic polymer-induced aggregation of gold nanoparticles (AuNPs). The principle of this assay is very classical. The aggregation of AuNPs was induced by the concentration of cationic polymer, which is controlled by specific recognition of aptamer with BPA and the reaction of aptamer and cationic polymer forming "duplex" structure. This method enables colorimetric detection of BPA with selectivity and a detection limit of 1.50 nM. In addition, this colorimetric method was successfully used to determine spiked BPA in tap water and river water samples.
在本研究中，基于双酚A（BPA）特异性适配体和阳离子聚合物诱导的金纳米颗粒（AuNPs）聚集，开发了一种比色法来检测双酚A。该检测方法的原理非常经典。阳离子聚合物的浓度诱导AuNPs聚集，而阳离子聚合物的浓度由适配体与BPA的特异性识别以及适配体与阳离子聚合物形成“双链体”结构的反应所控制。该方法能够选择性地比色检测双酚A，检测限为1.50 nM。此外，该比色法成功用于测定自来水和河水样品中添加的双酚A。
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