BIAN: A Multilayer Microfluidic-Based Tissue-Mimicking Phantom for Near-Infrared Imaging.

Near-infrared spectroscopy (NIRS) is a non-invasive optical method for monitoring cerebral oxygenation. Changes in regional blood flow and oxygenation due to neurovascular coupling are important biomarkers of neuronal activation. So far, there has been little research on multilayer tissue phantoms with tuneable blood flow, blood volume, and optical properties to simulate local changes in oxygenation at different depths. The aim of this study is to design, fabricate and characterize a complex dynamic phantom based on multilayer microfluidics with controllable blood flow, blood volume, and optical properties for testing NIRS instruments. We developed a phantom prototype with two microfluidic chips embedded at two depths inside a solid silicone phantom to mimic the vessels in the scalp and in the cortex. To simulate the oxygenation and perfusion of tissue, a solution with blood-like optical properties was sent into the microchannels by a pump with a programmable pressure controller. The pressure adjusted the volume of the microfluidic chips representing a distension of blood vessels. The optical changes in the superficial and deep layers were measured by a commercially available frequency domain NIRS instrument. The NIRS successfully detected the changes in light intensity elicited by the changes in the pressure input to the two layers. In conclusion, the microfluidics-based imaging phantom was successfully designed and fabricated and mimics brain functional activity. This technique has great potential for testing other optical devices, e.g., diffuse correlation spectroscopy, pulse oximetry, and optical coherence tomography.