无血清生物测定中TNF-α和TNF-β细胞溶解生物活性的比较。
Comparison of TNF-alpha and TNF-beta cytolytic biological activities in a serum-free bioassay.
作者信息
Kramer S M, Carver M E, Apperson S M
出版信息
Lymphokine Res. 1986;5 Suppl 1:S139-43.
PMID:3784609
Abstract
The effect of temperature and serum-free conditions on the cytolytic biological activities of TNF-alpha and TNF-beta were evaluated using murine connective tissue L-M as the target cell line. Serum-free assay medium and a 39 degrees C incubation temperature substantially enhanced the activity of both cytokines. L-M cells were 3 to 4 times more sensitive to cytolysis by TNF-beta than TNF-alpha under serum-free assay conditions.
摘要
以小鼠结缔组织L-M作为靶细胞系，评估温度和无血清条件对肿瘤坏死因子-α（TNF-α）和肿瘤坏死因子-β（TNF-β）细胞溶解生物活性的影响。无血清测定培养基和39℃的孵育温度显著增强了两种细胞因子的活性。在无血清测定条件下，L-M细胞对TNF-β介导的细胞溶解作用的敏感性比对TNF-α的敏感性高3至4倍。
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