Lombrozo L, Anderson T J, Kanaske K, Hollister L E
J Chromatogr. 1980 Jan 11;181(1):1-10. doi: 10.1016/s0378-4347(00)81262-1.
Urinary 3-methoxy-4-hydroxyphenylglycol has been reliably assayed on a routine basis using an electron-capture detector method. Modifications of previous procedures include simplifcation of extraction and derivatization of urine, inclusion of an internal standard, prevention of losses during concentration, use of each urine as its own standard, and better chromatographic resolution by lengthening of columns and programming temperature. The assay shows a coefficient of variation of 3.1%.
尿中3-甲氧基-4-羟基苯乙二醇已通过电子捕获检测法在常规基础上进行可靠测定。对先前程序的改进包括简化尿液提取和衍生化过程、加入内标、防止浓缩过程中的损失、将每份尿液用作自身标准以及通过延长色谱柱和程序升温实现更好的色谱分离度。该测定法的变异系数为3.1%。
