Biondi P A, Niu-Fan G, Secchi C
Clin Chim Acta. 1982 May 6;121(1):79-86. doi: 10.1016/0009-8981(82)90213-3.
In order to develop a gas chromatographic method with flame ionization detection for the analysis of urinary 3-methoxy-4-hydroxyphenylethyleneglycol sulphate, a new procedure for its isolation has been introduced. The conjugated metabolite has been extracted by an ion-pair technique using tetrabutyl ammonium as counter ion and dichloromethane as organic solvent. Hydrolysis and derivatization of 3-methoxy-4-hydroxyphenylethyleneglycol sulphate have been finally performed by treatment with heptafluorobutyric anhydride. Each step of the procedure, applied to the analysis of urine samples both from normal subjects and from patients with neuroblastoma, has been discussed.
为开发一种采用火焰离子化检测的气相色谱法来分析尿中硫酸3-甲氧基-4-羟基苯乙二醇，已引入一种新的分离方法。使用四丁基铵作为反离子、二氯甲烷作为有机溶剂，通过离子对技术提取共轭代谢物。硫酸3-甲氧基-4-羟基苯乙二醇的水解和衍生化最终通过用七氟丁酸酐处理来进行。已讨论了该方法应用于正常受试者和神经母细胞瘤患者尿液样本分析的每一步骤。
