Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.
Institute of Photonics and Electronics of the Czech Academy of Sciences, Chaberska 1014/57, 18200, Prague, Czech Republic.
Mikrochim Acta. 2023 Oct 17;190(11):442. doi: 10.1007/s00604-023-05999-2.
Electrochemical methods can be used not only for the sensitive analysis of proteins but also for deeper research into their structure, transport functions (transfer of electrons and protons), and sensing their interactions with soft and solid surfaces. Last but not least, electrochemical tools are useful for investigating the effect of an electric field on protein structure, the direct application of electrochemical methods for controlling protein function, or the micromanipulation of supramolecular protein structures. There are many experimental arrangements (modalities), from the classic configuration that works with an electrochemical cell to miniaturized electrochemical sensors and microchip platforms. The support of computational chemistry methods which appropriately complement the interpretation framework of experimental results is also important. This text describes recent directions in electrochemical methods for the determination of proteins and briefly summarizes available methodologies for the selective labeling of proteins using redox-active probes. Attention is also paid to the theoretical aspects of electron transport and the effect of an external electric field on the structure of selected proteins. Instead of providing a comprehensive overview, we aim to highlight areas of interest that have not been summarized recently, but, at the same time, represent current trends in the field.
电化学方法不仅可用于蛋白质的灵敏分析，还可用于更深入地研究其结构、传输功能（电子和质子的转移），以及感测其与软质和硬质表面的相互作用。最后但同样重要的是，电化学工具可用于研究电场对蛋白质结构的影响、直接应用电化学方法控制蛋白质功能或对超分子蛋白质结构进行微操作。有许多实验装置（模式），从经典的电化学池工作配置到微型化电化学传感器和微芯片平台。计算化学方法的支持也很重要，它适当地补充了实验结果的解释框架。本文描述了用于测定蛋白质的电化学方法的最新方向，并简要总结了使用氧化还原活性探针选择性标记蛋白质的现有方法。本文还关注电子传输的理论方面以及外部电场对选定蛋白质结构的影响。我们的目的不是提供全面的概述，而是强调最近没有被总结的但同时代表该领域当前趋势的感兴趣领域。
