Monstein H J, Folkesson R, Terenius L
Life Sci. 1986 Dec 8;39(23):2237-41. doi: 10.1016/0024-3205(86)90402-9.
Total RNA has been prepared from human leukocytes from patients with chronic lymphoblastic leukemia (CLL) as well as from post mortem human caudate nucleus, hypothalamus, cerebellum and cerebral cortex. Dot-blot and Northern blot analysis, using a human proenkephalin A clone and SP-6 derived "complementary" proenkephalin A RNA respectively, revealed the existence of proenkephalin A-like RNA:s in CLL-leukocytes with the same characteristics as in caudate nucleus, hypothalamus, and cortex. Furthermore, RIA and Western blot analysis confirmed that immunoreactive pro-enkephalin A activity is present in human CLL-leukocytes. The progress in DNA recombinant technology has allowed the study of opioid peptide regulation at the transcriptional and translational-posttranslational level. Studies on the distribution and quantitation of preproenkephalin A mRNA in bovine, rat and human central nervous system (CNS) have recently been reported. Different opioid peptides, related to the enkephalins, dynorphins and beta-endorphin have also been detected in tissues outside the CNS including the adrenal medulla and in pheochromocytomas. Northern blot analysis and cDNA-cloning confirmed that the proenkephalin A gene is indeed expressed in these tissues. Proenkephalin A derived peptides are potentially significant in nervous disorders. We have chosen to investigate whether the corresponding gene is expressed not only in CNS-tissues but also in human leukocytes, cells readily obtained in individual patients.
已从慢性淋巴细胞白血病（CLL）患者的人白细胞以及人死后的尾状核、下丘脑、小脑和大脑皮层中制备了总RNA。分别使用人前脑啡肽原A克隆和SP - 6衍生的“互补”前脑啡肽原A RNA进行斑点印迹和Northern印迹分析，结果显示CLL白细胞中存在与尾状核、下丘脑和皮层中具有相同特征的前脑啡肽原A样RNA。此外，放射免疫分析（RIA）和蛋白质免疫印迹分析证实人CLL白细胞中存在免疫反应性前脑啡肽原A活性。DNA重组技术的进展使得在转录和翻译后水平研究阿片肽调节成为可能。最近有报道对牛、大鼠和人的中枢神经系统（CNS）中前脑啡肽原A mRNA的分布和定量进行了研究。在中枢神经系统以外的组织包括肾上腺髓质和嗜铬细胞瘤中也检测到了与脑啡肽、强啡肽和β-内啡肽相关的不同阿片肽。Northern印迹分析和cDNA克隆证实前脑啡肽原A基因确实在这些组织中表达。前脑啡肽原A衍生的肽在神经疾病中可能具有重要意义。我们选择研究相应基因是否不仅在中枢神经系统组织中表达，而且在个体患者易于获取的人白细胞中表达。
