Monstein H J, Geijer T
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala.
Biosci Rep. 1988 Jun;8(3):255-61. doi: 10.1007/BF01115042.
Total RNA from post mortem human caudate nucleus, cerebellum, cerebral cortex and pheochromocytoma tissues has been prepared. Northern blot analysis, using a single-stranded human proenkephalin A antisense probe (cRNA), revealed the existence of two different proenkephalin A-like sequences in the human caudate nucleus and pheochromocytoma RNA extracts of approximately 1400 and 1000 nucleotides in length respectively, whereas no specific RNA bands could be detected in the cortex and only the 1400 nucleotide band was present in the cerebellum. Under highly stringent hybridization conditions, the proenkephalin A-like RNA bands still appear, indicating that the detected RNA species have either identical or a closely related sequence to that of the well-characterized human proenkephalin A mRNA sequence.
已制备来自人类尾状核、小脑、大脑皮层和嗜铬细胞瘤组织的死后总RNA。使用单链人脑啡肽原A反义探针（cRNA）进行的Northern印迹分析显示，在人类尾状核和嗜铬细胞瘤RNA提取物中存在两种不同的脑啡肽原A样序列，长度分别约为1400和1000个核苷酸，而在皮层中未检测到特异性RNA条带，且在小脑中仅存在1400个核苷酸的条带。在高度严格的杂交条件下，脑啡肽原A样RNA条带仍然出现，表明检测到的RNA种类与已充分表征的人类脑啡肽原A mRNA序列具有相同或密切相关的序列。
