Int J Oral Maxillofac Implants. 2023 Oct 17;38(5):1025-1033. doi: 10.11607/jomi.9994.
To evaluate the effect of ultraviolet-C light (UVC) photofunctionalization treatment time on the biologic activity of airborne particle-abraded and acid-etched (SLA) titanium surfaces and to analyze its physical and chemical mechanisms.
SLA titanium was treated with UVC light for different lengths of time (10 minutes, 20 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, and 24 hours), and then changes to its surface characteristics were evaluated via electron microscope scanning, X-ray photoelectron spectroscopy (XPS), water contact angle measurement, and zeta potential measurement. The effect of UVC photofunctionalization on the biologic processes of SLA titanium surfaces was assessed by analyzing the bovine serum albumin adsorption, adhesion, proliferation, morphology, and alkaline phosphatase activity of MG-63 cells.
UVC irradiation did not change the topography of SLA titanium surfaces. As treatment times increased, the water contact angle decreased from 120 degrees to 0 degrees, and the hydrocarbon content decreased. Zeta potential testing showed increased surface potential of photofunctionalized titanium. In vitro testing showed that cell adhesion, proliferation, morphology, and alkaline phosphate (ALP) activity on titanium surfaces were significantly improved by UVC photofunctionalization.
UVC photofunctionalization can improve the biologic activity of SLA titanium surfaces by removing hydrocarbons and increasing the surface potential of titanium.
评估紫外线-C 光（UVC）光功能化处理时间对空气中颗粒磨损和酸蚀（SLA）钛表面生物活性的影响，并分析其物理和化学机制。
SLA 钛经 UVC 光处理不同时间（10 分钟、20 分钟、30 分钟、1 小时、2 小时、4 小时和 24 小时），然后通过电子显微镜扫描、X 射线光电子能谱（XPS）、水接触角测量和zeta 电位测量评估其表面特性的变化。通过分析牛血清白蛋白在 SLA 钛表面的吸附、黏附、增殖、形态和碱性磷酸酶活性，评估 UVC 光功能化对 SLA 钛表面生物学过程的影响。
UVC 照射未改变 SLA 钛表面的形貌。随着处理时间的增加，水接触角从 120 度降至 0 度，碳氢化合物含量降低。Zeta 电位测试显示光功能化钛的表面电位增加。体外试验表明，UVC 光功能化可通过去除碳氢化合物和增加钛表面的表面电位，显著改善钛表面的细胞黏附、增殖、形态和碱性磷酸酶（ALP）活性。
UVC 光功能化可以通过去除碳氢化合物和增加钛表面的表面电位来提高 SLA 钛表面的生物活性。
