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从山茶花的 RNA-seq 数据中开发基因 SSR 标记资源及其在山茶属中的应用。

Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia.

机构信息

Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, 430075, China.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

出版信息

Sci Rep. 2021 May 10;11(1):9919. doi: 10.1038/s41598-021-89350-w.

DOI:10.1038/s41598-021-89350-w
PMID:33972624
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8110538/
Abstract

Camellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeats (SSRs) publicly available for plants in this genus is insufficient. In this study, a total of 28,854 potential SSRs were identified with a frequency of 4.63 kb. A total of 172 primer pairs were synthesized and preliminarily screened in 10 C. japonica accessions, and of these primer pairs, 111 were found to be polymorphic. Fifty-one polymorphic SSR markers were randomly selected to perform further analysis of the genetic relationships of 89 accessions across the genus Camellia. Cluster analysis revealed major clusters corresponding to those based on taxonomic classification and geographic origin. Furthermore, all the genotypes of C. japonica separated and consistently grouped well in the genetic structure analysis. The results of the present study provide high-quality SSR resources for molecular genetic breeding studies in camellia plants.

摘要

山茶是山茶科的一个花卉植物属，该属的几个物种具有经济重要性。尽管在过去十年中，为山茶属的分子辅助育种开发了大量的分子标记，但该属植物可用的简单重复序列 (SSR) 的数量还不够。在这项研究中，共鉴定出 28854 个潜在的 SSR，其频率为 4.63 kb。总共合成了 172 对引物，并在 10 个山茶品种中进行了初步筛选，其中 111 对引物表现出多态性。随机选择 51 个多态性 SSR 标记，对山茶属 89 个品种的遗传关系进行进一步分析。聚类分析显示，主要聚类与基于分类学和地理起源的聚类相对应。此外，在遗传结构分析中，所有的山茶品种基因型都分离并始终很好地分组。本研究的结果为山茶植物的分子遗传育种研究提供了高质量的 SSR 资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/0c4ef0f75691/41598_2021_89350_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/18e07ae894fd/41598_2021_89350_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/49f1b8c5a758/41598_2021_89350_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/39ffc86972bd/41598_2021_89350_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/0dfa3bf03e4b/41598_2021_89350_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/e657aa3fd32f/41598_2021_89350_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/0c4ef0f75691/41598_2021_89350_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/18e07ae894fd/41598_2021_89350_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/49f1b8c5a758/41598_2021_89350_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/39ffc86972bd/41598_2021_89350_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/0dfa3bf03e4b/41598_2021_89350_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/e657aa3fd32f/41598_2021_89350_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/8110538/0c4ef0f75691/41598_2021_89350_Fig6_HTML.jpg

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