Peticca Aurelie, Fodil Mostefa, Gateau Helene, Mouget Jean-Luc, Sabot Francois, Chenais Benoit, Casse Nathalie
BiOSSE (Biology of Organisms: Stress, Health, Environment), UFR Sciences et Techniques, Le Mans Universite, Le Mans, France.
DIADE, University of Montpellier, CIRAD, Montferrier-sur-Lez, France.
Mitochondrial DNA B Resour. 2023 Oct 13;8(10):1092-1096. doi: 10.1080/23802359.2023.2268747. eCollection 2023.
The first completed, circular mitochondrial genome and the first draft, linear chloroplastic genome of the blue diatom (Simonsen 1974, ) were assembled from Illumina and PacBio sequencing. The mitochondrial genome was composed of 38,696 bases and contained 64 genes, including 31 protein-coding genes (CDS), 2 ribosomal RNA (rRNA) genes and 23 transfer RNA (tRNA) genes. For the chloroplast, the genome was composed of 130,200 bases with 169 genes (131 CDS, 6 rRNA genes, 31 tRNA genes, and 1 transfer messenger RNA gene). Phylogenetic trees, using the maximum-likehood method and partial genes currently available for and other diatom species, suggested the proximity of all the strains/isolates and the possibility of using these genomes as future references.
首个完整的环状线粒体基因组以及首个蓝硅藻(西蒙森,1974年)的线性叶绿体基因组草图是通过Illumina和PacBio测序组装而成的。线粒体基因组由38,696个碱基组成,包含64个基因,其中包括31个蛋白质编码基因(CDS)、2个核糖体RNA(rRNA)基因和23个转运RNA(tRNA)基因。对于叶绿体而言,基因组由130,200个碱基组成,有169个基因(131个CDS、6个rRNA基因、31个tRNA基因和1个转运信使RNA基因)。利用最大似然法以及当前可用于该蓝硅藻和其他硅藻物种的部分基因构建的系统发育树表明,所有该蓝硅藻菌株/分离株之间亲缘关系较近,并且有可能将这些基因组用作未来的参考。