Rostain William, Shen Shensi, Cordero Teresa, Rodrigo Guillermo, Jaramillo Alfonso
Warwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK.
Institute of Systems and Synthetic Biology, CNRS-Université d'Évry Val-d'Essonne, 91000 Évry, France.
Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020.
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in . We showed that the resulting riboregulator can -activate gene expression by interacting with a -repressed messenger RNA. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker, and we modeled this novel posttranscriptional mechanism. This first reported example of a circular RNA regulating gene expression in adds to an increasing repertoire of RNA synthetic biology parts, and it highlights that topological molecules can play a role in the case of prokaryotic regulation.
不同形状和大小的RNA，无论是天然的还是合成的，都可以在原核生物和真核生物中调节基因表达。环状RNA最近似乎比以前认为的更为普遍，但其在原核生物中的作用仍然难以捉摸。在这里，通过在I组重排内含子-外显子核酶中插入一个核糖调节序列，我们创建了一种小的非编码RNA，它可以自我剪接以在 中产生一个环状核糖调节因子。我们表明，产生的核糖调节因子可以通过与一个被 -抑制的信使RNA相互作用来激活基因表达。我们用荧光报告基因和抗生素抗性标记对该系统进行了表征，并对这种新的转录后机制进行了建模。这是首次报道的环状RNA在 中调节基因表达的例子，它增加了越来越多的RNA合成生物学元件，并且突出了拓扑分子在原核生物调节中可以发挥作用。
