微生物细胞工厂中的转运体工程提升生物制造能力。
Transporter Engineering in Microbial Cell Factory Boosts Biomanufacturing Capacity.
作者信息
Lv Xiaodong, Xue Haijie, Qin Lei, Li Chun
机构信息
Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China.
Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China.
出版信息
Biodes Res. 2022 Jun 15;2022:9871087. doi: 10.34133/2022/9871087. eCollection 2022.
Microbial cell factories (MCFs) are typical and widely used platforms in biomanufacturing for designing and constructing synthesis pathways of target compounds in microorganisms. In MCFs, transporter engineering is especially significant for improving the biomanufacturing efficiency and capacity through enhancing substrate absorption, promoting intracellular mass transfer of intermediate metabolites, and improving transmembrane export of target products. This review discusses the current methods and strategies of mining and characterizing suitable transporters and presents the cases of transporter engineering in the production of various chemicals in MCFs.
微生物细胞工厂（MCFs）是生物制造中典型且广泛应用的平台，用于设计和构建微生物中目标化合物的合成途径。在微生物细胞工厂中，转运体工程对于提高生物制造效率和能力尤为重要，其方式包括增强底物吸收、促进中间代谢物的细胞内传质以及改善目标产物的跨膜输出。本文综述了挖掘和表征合适转运体的当前方法和策略，并介绍了微生物细胞工厂中各种化学品生产中转运体工程的案例。
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