Operative Unit of Clinical Microbiology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.
Department of Public Health of Emilia-Romagna Region, Bologna, Italy.
Microb Drug Resist. 2024 Jan;30(1):50-54. doi: 10.1089/mdr.2023.0150. Epub 2023 Oct 17.
is a concerning pathogen in health care due to its ability to spread in medical settings. In this study, we characterized the genome of three clinical isolates collected in the Emilia-Romagna region of Northeastern Italy from January 2020 to May 2021. Whole-genome sequencing was performed using Illumina iSeq 100 and Oxford Nanopore MinION systems. Genomes were assembled with Flye. Phylogenetic analysis was carried out with RaxML. The ERG, , and genes were examined for known substitutions associated with resistance to azoles and caspofungin using Diamond. All three isolates belonged to clade I (South Asian lineage) and showed high minimum inhibitory concentrations for fluconazole. Two of the three isolates were closely related to the first Italian index case of occurred in the 2019 and carried similar mutations associated to azole resistance. The third isolate showed a greater phylogenetic distance from these strains and had a different genetic determinant not previously seen in Italy. Our data suggest that two clinical isolates may have been epidemiologically related to the first outbreak previously observed in Italy, while the remaining isolate may have originated from a different source. Further research is needed to understand transmission and resistance and to control its spread.
是一种令人担忧的病原体，因为它能够在医疗环境中传播。在这项研究中，我们对 2020 年 1 月至 2021 年 5 月在意大利东北部艾米利亚-罗马涅地区采集的三个临床分离株的基因组进行了特征描述。使用 Illumina iSeq 100 和 Oxford Nanopore MinION 系统进行全基因组测序。使用 Flye 进行基因组组装。使用 RaxML 进行系统发育分析。使用 Diamond 检查 ERG、、和 基因中与唑类和卡泊芬净耐药相关的已知取代。这三个 分离株均属于 I 组（南亚谱系），对氟康唑的最低抑菌浓度较高。三个分离株中的两个与 2019 年意大利首例病例密切相关，携带与唑类耐药相关的类似突变。第三个分离株与这些菌株的系统发育距离较远，具有意大利以前未见的不同遗传决定因素。我们的数据表明，两个 临床分离株可能与意大利之前观察到的首次暴发在流行病学上有关，而其余的分离株可能来自不同的来源。需要进一步研究以了解 的传播和耐药性，并控制其传播。
