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在特定人类DNA序列中检测紫外线嘌呤光产物。

Detection of UV purine photoproducts in a defined sequence of human DNA.

作者信息

Gallagher P E, Duker N J

出版信息

Mol Cell Biol. 1986 Feb;6(2):707-9. doi: 10.1128/mcb.6.2.707-709.1986.

DOI:10.1128/mcb.6.2.707-709.1986
PMID:3785156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367562/
Abstract

The UV-irradiated, 3'-end-labeled, 92-base-pair terminus of the human alphoid sequence was incubated with purified endonuclease v. Previously unreported photoproducts were incised at purine loci. These were not pyrimidine photodimers, 6-4'-(pyrimidin-2'-one)-pyrimidines, base loss sites, or ring-opened purines. Therefore, purine-containing photoproducts, possibly dimers, were incised by the enzyme preparation.

摘要

将经紫外线照射、3' 端标记的 92 个碱基对的人类α卫星序列末端与纯化的核酸内切酶 v 一起孵育。以前未报道的光产物在嘌呤位点被切割。这些不是嘧啶光二聚体、6-4'-(嘧啶-2'-酮)-嘧啶、碱基缺失位点或开环嘌呤。因此，含嘌呤的光产物，可能是二聚体，被该酶制剂切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a1c/367562/c4dc7bdc2f2c/molcellb00086-0372-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a1c/367562/9e6e1d60a620/molcellb00086-0372-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a1c/367562/c4dc7bdc2f2c/molcellb00086-0372-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a1c/367562/9e6e1d60a620/molcellb00086-0372-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a1c/367562/c4dc7bdc2f2c/molcellb00086-0372-b.jpg

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1
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本文引用的文献

1
UV-induced mutation hotspots occur at DNA damage hotspots.紫外线诱导的突变热点出现在DNA损伤热点处。
Nature. 1982 Jul 8;298(5870):189-92. doi: 10.1038/298189a0.
2
Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.紫外线诱导的人类DNA特定序列损伤的分布：嘧啶核苷 - 胞苷序列处碱敏感损伤的检测
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3
Formation of an adenine-thymine photoadduct in the deoxydinucleoside monophosphate d(TpA) and in DNA.
独特的胸腺嘧啶 - 胸腺嘧啶二聚体或胸腺嘧啶 - 胸腺嘧啶嘧啶酮（6 - 4）光产物在哺乳动物细胞中的诱变性。
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4
HPLC photofingerprinting of conformational peculiarities and transitions in oligonucleotide duplexes.寡核苷酸双链体构象特性及转变的高效液相色谱光指纹图谱分析
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5
The isolation and characterisation of a new type of dimeric adenine photoproduct in UV-irradiated deoxyadenylates.紫外线照射脱氧腺苷酸中新型二聚体腺嘌呤光产物的分离与表征
Nucleic Acids Res. 1987 Feb 11;15(3):1199-216. doi: 10.1093/nar/15.3.1199.
6
Photoalkylated DNA and ultraviolet-irradiated DNA are incised at cytosines by endonuclease III.经光烷基化的DNA和紫外线照射的DNA会被核酸内切酶III在胞嘧啶处切割。
Nucleic Acids Res. 1986 Aug 26;14(16):6621-31. doi: 10.1093/nar/14.16.6621.
7
The specificity of UV-induced mutations at an endogenous locus in mammalian cells.哺乳动物细胞内源性位点紫外线诱导突变的特异性。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9103-7. doi: 10.1073/pnas.84.24.9103.
8
Photoproduct frequency is not the major determinant of UV base substitution hot spots or cold spots in human cells.光产物频率并非人类细胞中紫外线碱基替换热点或冷点的主要决定因素。
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3782-6. doi: 10.1073/pnas.84.11.3782.
9
UV-induced photoproducts of 5-methylcytosine in a DNA sequence context.DNA序列环境中5-甲基胞嘧啶的紫外线诱导光产物。
Nucleic Acids Res. 1988 Apr 25;16(8):3327-40. doi: 10.1093/nar/16.8.3327.
10
A novel photoproduct of 2'-deoxyguanosine induced by acetone photosensitization: 8-(2,3,4-trihydroxybutyl)guanine.丙酮光敏化诱导产生的一种新型2'-脱氧鸟苷光产物：8-(2,3,4-三羟基丁基)鸟嘌呤。
Nucleic Acids Res. 1989 Feb 11;17(3):955-67. doi: 10.1093/nar/17.3.955.
在脱氧单磷酸二核苷d(TpA)和DNA中形成腺嘌呤-胸腺嘧啶光加合物。
Science. 1983 May 13;220(4598):723-5. doi: 10.1126/science.6836308.
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The photoreactivity of T-A sequences in oligodeoxyribonucleotides and DNA.寡脱氧核糖核苷酸和DNA中T-A序列的光反应活性
Nucleic Acids Res. 1984 Oct 25;12(20):7903-14. doi: 10.1093/nar/12.20.7903.
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Mutagenic action of monochromatic UV radiation in the solar range on human cells.
Mutat Res. 1984 Oct;129(1):103-10. doi: 10.1016/0027-5107(84)90128-3.
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Ultraviolet light repair and mutagenesis revisited.紫外线修复与诱变再探讨。
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Enzymic detection of uracil in a cloned and sequenced deoxyribonucleic acid segment.对一个克隆并测序的脱氧核糖核酸片段中尿嘧啶的酶促检测。
Biochemistry. 1983 Sep 13;22(19):4501-7. doi: 10.1021/bi00288a023.
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J Biol Chem. 1980 Dec 25;255(24):12047-50.
10
Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA.脱嘌呤/脱嘧啶内切核酸酶在DNA嘧啶二聚体及其他损伤修复中的作用
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4602-6. doi: 10.1073/pnas.77.8.4602.