Department of Electrical and Computer Engineering Boston University, Boston, MA, United States of America.
Department of Biomedical Engineering, Boston University, Boston, MA, United States of America.
PLoS One. 2023 Oct 18;18(10):e0286988. doi: 10.1371/journal.pone.0286988. eCollection 2023.
Illumination uniformity is a critical parameter for excitation and data extraction quality in widefield biological imaging applications. However, typical imaging systems suffer from spatial and spectral non-uniformity due to non-ideal optical elements, thus require complex solutions for illumination corrections. We present Effective Uniform Color-Light Integration Device (EUCLID), a simple and cost-effective illumination source for uniformity corrections. EUCLID employs a diffuse-reflective, adjustable hollow cavity that allows for uniform mixing of light from discrete light sources and modifies the source field distribution to compensate for spatial non-uniformity introduced by optical components in the imaging system. In this study, we characterize the light coupling efficiency of the proposed design and compare the uniformity performance with the conventional method. EUCLID demonstrates a remarkable illumination improvement for multi-spectral imaging in both Nelsonian and Koehler alignment with a maximum spatial deviation of ≈ 1% across a wide field-of-view.
照明均匀度是宽场生物成像应用中激发和数据提取质量的关键参数。然而，由于非理想的光学元件，典型的成像系统会受到空间和光谱不均匀性的影响，因此需要复杂的解决方案来进行照明校正。我们提出了有效的均匀彩色光集成装置（EUCLID），这是一种简单且具有成本效益的照明源，可用于均匀性校正。EUCLID 采用漫反射、可调的空心腔，可实现来自离散光源的光的均匀混合，并修改光源场分布，以补偿成像系统中光学元件引入的空间不均匀性。在这项研究中，我们对所提出设计的光耦合效率进行了表征，并将均匀性性能与传统方法进行了比较。EUCLID 展示了在纳尔逊和科勒两种对准方式下的多光谱成像的显著照明改进，在宽视场中最大空间偏差约为 1%。
