Bieberich C, Scangos G, Tanaka K, Jay G
Mol Cell Biol. 1986 Apr;6(4):1339-42. doi: 10.1128/mcb.6.4.1339-1342.1986.
The major histocompatibility complex class I genes play an essential role in the immune presentation of aberrant cells. To gain further insight into the regulation of the expression of these class I genes and to better define the functions of their protein products, we made use of the technique of gene transfer into the germ line of inbred mice. With the use of locus-specific DNA probes, we observed that a transgenic class I gene was expressed in a tissue-dependent fashion analogous to that of an endogenous class I gene. In addition, the level of expression of the transgenic gene was substantially higher that that of the endogenous gene. The availability of transgenic mice properly expressing a foreign murine class I gene provides a unique system to further define the role of the class I antigens in the maturation of the immune response and in determining the malignant and metastatic phenotypes of tumor cells.
主要组织相容性复合体I类基因在异常细胞的免疫呈递中起重要作用。为了进一步深入了解这些I类基因表达的调控机制，并更好地确定其蛋白质产物的功能，我们利用了将基因导入近交系小鼠种系的技术。通过使用位点特异性DNA探针，我们观察到转基因I类基因以类似于内源性I类基因的组织依赖性方式表达。此外，转基因基因的表达水平显著高于内源性基因。正确表达外源小鼠I类基因的转基因小鼠的获得，为进一步确定I类抗原在免疫反应成熟以及在决定肿瘤细胞的恶性和转移表型中的作用提供了一个独特的系统。
