Lieber T, Weisser K, Childs G
Mol Cell Biol. 1986 Jul;6(7):2602-12. doi: 10.1128/mcb.6.7.2602-2612.1986.
We analyzed the histone mRNA population found in several adult tissues of the sea urchin Strongylocentrotus purpuratus and in testis of Lytechinus pictus. Unique species of H1 and H2b mRNAs encoding the sperm-specific histone subtypes can be found exclusively in testis RNA. S. purpuratus contains two distinct testis-specific H1 transcripts, while L. pictus contains one such transcript. Each of these mRNAs is larger than either early or late embryonic H1 mRNAs. Other somatic adult tissues contain transcripts derived from members of the late embryonic H1 histone gene family. S. purpuratus contains one H2b transcript found exclusively in testis, while L. pictus contains two such H2b mRNAs. Similarly, in tissues other than testis, late H2b transcripts were found. While there is no sperm-specific H2a protein, a limited set of late histone H2a genes encoding primarily the H2a-beta subtype is expressed in testis. The majority of the H2a protein found in diploid adult tissues is also the H2a-beta subtype; however, the size of the H2a transcripts differs between testis and other tissues. We conclude that different members of the late H2a gene family are differentially expressed in embryos and adult tissues. We prepared and characterized cDNA clones encoding the sperm-specific H2b protein as well as the H2a-beta protein found in testis.
我们分析了在紫海胆（Strongylocentrotus purpuratus）的几种成体组织以及花斑毒棘海胆（Lytechinus pictus）的睾丸中发现的组蛋白mRNA群体。编码精子特异性组蛋白亚型的H1和H2b mRNA的独特种类仅能在睾丸RNA中找到。紫海胆含有两种不同的睾丸特异性H1转录本，而花斑毒棘海胆含有一种这样的转录本。这些mRNA中的每一种都比早期或晚期胚胎H1 mRNA更大。其他成体体细胞组织含有源自晚期胚胎H1组蛋白基因家族成员的转录本。紫海胆含有一种仅在睾丸中发现的H2b转录本，而花斑毒棘海胆含有两种这样的H2b mRNA。同样，在睾丸以外的组织中也发现了晚期H2b转录本。虽然不存在精子特异性H2a蛋白，但一组有限的主要编码H2a-β亚型的晚期组蛋白H2a基因在睾丸中表达。在二倍体成体组织中发现的大多数H2a蛋白也是H2a-β亚型；然而，睾丸和其他组织中H2a转录本的大小有所不同。我们得出结论，晚期H2a基因家族的不同成员在胚胎和成人组织中差异表达。我们制备并鉴定了编码精子特异性H2b蛋白以及在睾丸中发现的H2a-β蛋白的cDNA克隆。
