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从太平洋紫海胆中分离并鉴定编码睾丸特异性组蛋白H2B-2的基因。

Isolation and characterization of the gene encoding the testis specific histone protein H2B-2 from the sea urchin Lytechinus pictus.

作者信息

Lai Z C, Childs G

出版信息

Nucleic Acids Res. 1986 Sep 11;14(17):6845-56. doi: 10.1093/nar/14.17.6845.

DOI:10.1093/nar/14.17.6845
PMID:3763394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311703/
Abstract

We have cloned and characterized the gene encoding a sperm specific H2B-2 histone subtype from the sea urchin L. pictus. The gene is not clustered with any other histone genes. However, it resembles other histone genes in many respects. The gene contains no intervening sequences or polyadenylation signals. Like other histone genes it contains the conserved 3' hairpin loop sequence and CAAGAAAGA box necessary for the processing of the 3' end of the histone transcript. Upstream of the gene in addition to the TATA box, and two copies of inverted CCAAT Boxes is a conserved sequence element found in many H2B genes. Unlike most histone genes, the H2B-2 gene is expressed in a sex and tissue specific manner only in testis. The sperm H2B-2 subtype encoded by this gene is 142 amino acids in length. The larger size of this H2B protein is accounted for by residues in the N-terminus of the protein consisting of a series of pentapeptide repeats typical of this histone subtype.

摘要

我们已经克隆并鉴定了来自海胆光棘球海胆（Lytechinus pictus）的编码精子特异性H2B - 2组蛋白亚型的基因。该基因不与任何其他组蛋白基因成簇。然而，它在许多方面类似于其他组蛋白基因。该基因不包含内含序列或聚腺苷酸化信号。与其他组蛋白基因一样，它包含保守的3'发夹环序列和CAAGAAAGA框，这对于组蛋白转录本3'末端的加工是必需的。在该基因上游，除了TATA框和两个反向CCAAT框的拷贝外，还有一个在许多H2B基因中发现的保守序列元件。与大多数组蛋白基因不同，H2B - 2基因仅在睾丸中以性别和组织特异性方式表达。由该基因编码的精子H2B - 2亚型长度为142个氨基酸。这种H2B蛋白较大的尺寸是由该蛋白N末端的残基造成的，这些残基由一系列这种组蛋白亚型典型的五肽重复序列组成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aac/311703/b99f32794dfc/nar00286-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aac/311703/0c8d4a20ac84/nar00286-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aac/311703/b99f32794dfc/nar00286-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aac/311703/0c8d4a20ac84/nar00286-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aac/311703/b99f32794dfc/nar00286-0078-a.jpg

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引用本文的文献

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2
A comprehensive compilation and alignment of histones and histone genes.组蛋白和组蛋白基因的全面汇编与比对。
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3
SPKK, a new nucleic acid-binding unit of protein found in histone.

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A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.一种蛋白质在三个特定位点与卫星DNA重复序列结合，这些位点在核小体中会通过DNA折叠而相互靠近。
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