Liang J C, Sherron D A, Johnston D
Mutat Res. 1986 Dec;163(3):285-97. doi: 10.1016/0027-5107(86)90027-8.
Aneuploidy represents the predominant type of chromosomal abnormality found in human newborns with birth defects. The concern that environmental agents may cause aneuploidy in germ cells has prompted development of assay systems for detection of potentially aneuploidy-producing agents. One of the most frequently used methods involves cytogenetic analysis of murine spermatogenic cells at the stages of meiotic metaphases. However, criteria for aneuploidy induction have not been standardized in this test system. Many investigators consider the ability of an agent to induce univalents an appropriate measure of its potential to induce aneuploidy. In the present study, the relationship between univalency and aneuploidy was examined in mouse spermatocytes after various mutagen treatments. 45 Swiss mice were treated with 4 different agents; viz., adriamycin, vinblastine sulfate, cytosine arabinoside, and radiation (cobalt 60) and 10 untreated animals served as controls. From each animal, 50-200 MIs were examined for both sex-chromosomal and autosomal univalency (X-Y U and AU), and equal numbers of MIIs were examined for aneuploidy (hyperhaploidy). No significant correlations between univalency (either X-Y U or AU) and aneuploidy were found in the mutagen-treated mice; nor were they found in the untreated animals. These results indicate that induction of univalents by a mutagen is not necessarily predictive of the aneuploidy-inducing ability of this agent.
非整倍体是在患有出生缺陷的人类新生儿中发现的主要染色体异常类型。环境因素可能导致生殖细胞出现非整倍体这一担忧促使人们开发检测潜在致非整倍体因子的检测系统。最常用的方法之一涉及对处于减数分裂中期阶段的小鼠生精细胞进行细胞遗传学分析。然而，在这个检测系统中，非整倍体诱导的标准尚未标准化。许多研究人员认为，一种因子诱导单价体的能力是衡量其诱导非整倍体潜力的合适指标。在本研究中，在对小鼠精母细胞进行各种诱变处理后，研究了单价体与非整倍体之间的关系。45只瑞士小鼠用4种不同的试剂处理，即阿霉素、硫酸长春碱、阿糖胞苷和辐射（钴60），10只未处理的动物作为对照。从每只动物中，检查50 - 200个中期Ⅰ细胞的性染色体和常染色体单价体（X - Y单价体和常染色体单价体），并检查相同数量的中期Ⅱ细胞的非整倍体（超单倍体）。在诱变处理的小鼠中，未发现单价体（X - Y单价体或常染色体单价体）与非整倍体之间有显著相关性；在未处理的动物中也未发现。这些结果表明，诱变剂诱导单价体并不一定能预测该试剂诱导非整倍体的能力。
