Myles Krista M, Ragle James Matthew, Ward Jordan D
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064.
MicroPubl Biol. 2023 Oct 2;2023. doi: 10.17912/micropub.biology.000996. eCollection 2023.
NHR-23 is a nuclear hormone receptor transcription factor involved in molting, apical extracellular matrix structure, and spermatogenesis. To determine NHR-23 expression dynamics, we previously tagged the endogenous locus with a tag. To allow co-localization of NHR-23 with green fluorescent protein-tagged factors of interest, we generated an equivalent strain carrying an tag to produce a C-terminal fusion. Similar to the knock-in, NHR-23 ::mScarlet::3xMyc was expressed in seam and hypodermal cells, vulval precursor cells, and the spermatogenic germline. We also observed a diffuse NHR-23::mScarlet expression pattern in spermatids and residual bodies after NHR-23 ceased to localize on chromatin. Further examination of this novel localization may provide insight into NHR-23 regulation of spermatogenesis.
NHR-23是一种核激素受体转录因子，参与蜕皮、顶端细胞外基质结构和精子发生过程。为了确定NHR-23的表达动态，我们之前用一个标签标记了内源性位点。为了使NHR-23与绿色荧光蛋白标记的感兴趣因子共定位，我们构建了一个携带标签的等效品系以产生C末端融合。与敲入情况类似，NHR-23::mScarlet::3xMyc在接缝和皮下细胞、外阴前体细胞以及生精种系中表达。我们还观察到，在NHR-23停止定位于染色质后，精子细胞和残余体中出现了弥漫性的NHR-23::mScarlet表达模式。对这种新定位的进一步研究可能有助于深入了解NHR-23对精子发生的调控。
