Kim Seong Min, Forsburg Susan L
Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States.
University of Southern California, Los Angeles, California, United States.
MicroPubl Biol. 2023 Oct 2;2023. doi: 10.17912/micropub.biology.000974. eCollection 2023.
Replication stress can induce DNA synthesis outside of replicative S-phase. We have previously demonstrated that fission yeast cells stimulate DNA synthesis in G2-phase but not in M-phase in response to DNA alkylating agent MMS. In this study, we show that various DNA repair pathways, including translesion synthesis and break-induced replication contribute to post-replicative DNA synthesis. Checkpoint kinases, various repair and resection proteins, and multiple polymerases are also involved.
复制应激可诱导在复制性S期之外进行DNA合成。我们之前已经证明，裂殖酵母细胞在响应DNA烷化剂MMS时，会在G2期而非M期刺激DNA合成。在本研究中，我们表明包括跨损伤合成和断裂诱导复制在内的各种DNA修复途径有助于复制后DNA合成。检查点激酶、各种修复和切除蛋白以及多种聚合酶也参与其中。
