Zhu Jiawei, Wang Anli, Miao Xingguo, Ye Hui, Pan Shuo, Zhang Chengxi, Qian Qiuping, Su Feifei
Infectious Disease Department, Wenzhou Central Hospital Wenzhou 325099 Zhejiang People's Republic of China
Wenzhou Medical University Wenzhou 325000 Zhejiang People's Republic of China.
RSC Adv. 2023 Oct 17;13(43):30453-30461. doi: 10.1039/d3ra06034a. eCollection 2023 Oct 11.
Infectious wounds pose significant challenges due to their susceptibility to bacterial infections, hindering tissue repair. This study introduces gradient gelatin nanocomposite hydrogels for wound healing and antibacterial biofilm management. These hydrogels, synthesized UV light polymerization, incorporate copper-doped polydopamine nanoparticles (PDA-Cu) and GelMA (gelatin methacrylate). The hydrogels have a unique structure with a porous upper layer and a denser lower layer, ensuring superior swelling (over than 600%) and effective contact with bacterial biofilms. experiments demonstrate their remarkable antibacterial properties, inhibiting and biofilms by over 45% and 53%, respectively. This antibacterial action is attributed to the regulation of reactive oxygen species (ROS) production, an alternative mechanism to bacterial cell wall disruption. Moreover, the hydrogels exhibit high biocompatibility with mammalian cells, making them suitable for medical applications. evaluation in a rat wound infection model shows that the gradient hydrogel treatment effectively controls bacterial biofilm infections and accelerates wound healing. The treated wounds have smaller infected areas and reduced bacterial colony counts. Histological analysis reveals reduced inflammation and enhanced granulation tissue formation in treated wounds, highlighting the therapeutic potential of these gradient nanocomposite hydrogels. In summary, gradient gelatin nanocomposite hydrogels offer promising multifunctional capabilities for wound healing and biofilm-related infections, paving the way for innovative medical dressings with enhanced antibacterial properties and biocompatibility.
感染性伤口由于易受细菌感染而对组织修复造成重大挑战。本研究引入了用于伤口愈合和抗菌生物膜管理的梯度明胶纳米复合水凝胶。这些水凝胶通过紫外光聚合合成，包含铜掺杂的聚多巴胺纳米颗粒（PDA-Cu）和甲基丙烯酸明胶（GelMA）。水凝胶具有独特的结构，上层多孔，下层较致密，确保了优异的溶胀性（超过600%）并能与细菌生物膜有效接触。实验证明了它们卓越的抗菌性能，分别抑制浮游菌和生物膜超过45%和53%。这种抗菌作用归因于活性氧（ROS）产生的调节，这是一种不同于破坏细菌细胞壁的替代机制。此外，水凝胶与哺乳动物细胞具有高度生物相容性，使其适用于医学应用。在大鼠伤口感染模型中的评估表明，梯度水凝胶治疗有效地控制了细菌生物膜感染并加速了伤口愈合。治疗后的伤口感染面积更小，细菌菌落计数减少。组织学分析显示，治疗后的伤口炎症减轻，肉芽组织形成增强，突出了这些梯度纳米复合水凝胶的治疗潜力。总之，梯度明胶纳米复合水凝胶为伤口愈合和生物膜相关感染提供了有前景的多功能能力，为具有增强抗菌性能和生物相容性的创新医用敷料铺平了道路。
