Goerdt Lukas, Swain Thomas A, Kar Deepayan, McGwin Gerald, Berlin Andreas, Clark Mark E, Owsley Cynthia, Sloan Kenneth R, Curcio Christine A
Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Heersink School of Medicine, Birmingham, AL, USA.
Department of Ophthalmology, University of Bonn, Bonn, Germany.
Transl Vis Sci Technol. 2024 Dec 2;13(12):19. doi: 10.1167/tvst.13.12.19.
For structure-function research at the transition of aging to age-related macular degeneration, we refined the current consensus optical coherence tomography (OCT) nomenclature and evaluated a novel review software for investigational high-resolution OCT imaging (HR-OCT; <3 µm axial resolution).
Volume electron microscopy, immunolocalizations, histology, and investigational devices informed a refined OCT nomenclature for a custom ImageJ-based review tool to assess retinal band visibility. We examined effects on retinal band visibility of automated real-time averaging (ART) 9 and 100 (11 eyes of 10 healthy young adults), aging (10 young vs 22 healthy aged), and age-related macular degeneration (AMD; 22 healthy aged, 17 early (e)AMD, 15 intermediate (i)AMD). Intrareader reliability was assessed.
Bands not included in consensus nomenclature are now visible using HR-OCT: inner plexiform layer (IPL) 1-5, outer plexiform layer (OPL) 1-2, outer segment interdigitation zone 1-2 (OSIZ, including hyporeflective outer segments), and retinal pigment epithelium (RPE) 1-5. Cohen's kappa was 0.54-0.88 for inner and 0.67-0.83 for outer retinal bands in a subset of 10 eyes. IPL-3-5 and OPL-2 visibility benefitted from increased ART. OSIZ-2 and RPE-1,2,3,5 visibility was worse in aged eyes than in young eyes. OSIZ-1-2, RPE-1, and RPE-5 visibility decreased in eAMD and iAMD compared to healthy aged eyes.
We reliably identified 28 retinal bands using a novel review tool for HR-OCT. Image averaging improved inner retinal band visibility. Aging and AMD development impacted outer retinal band visibility.
Detailed knowledge of anatomic structures visible on OCT will enhance precision in research, including AI training and structure-function analyses.
为了在衰老向年龄相关性黄斑变性转变过程中进行结构-功能研究，我们完善了当前的光学相干断层扫描（OCT）共识命名法，并评估了一种用于研究性高分辨率OCT成像（HR-OCT；轴向分辨率<3µm）的新型图像分析软件。
通过体积电子显微镜、免疫定位、组织学和研究性设备，为基于ImageJ的自定义图像分析工具完善了OCT命名法，以评估视网膜条带的可视性。我们研究了自动实时平均（ART）9和100（10名健康年轻成年人的11只眼）、衰老（10名年轻人与22名健康老年人）以及年龄相关性黄斑变性（AMD；22名健康老年人、17名早期（e）AMD患者、15名中期（i）AMD患者）对视网膜条带可视性的影响。评估了阅片者内可靠性。
使用HR-OCT现在可以看到共识命名法中未包括的条带：内网状层（IPL）1-5、外网状层（OPL）1-2、外节指状交叉区1-2（OSIZ，包括低反射外节）和视网膜色素上皮（RPE）1-5。在10只眼的子集中，内视网膜条带的Cohen's kappa为0.54-0.88，外视网膜条带的Cohen's kappa为0.67-0.83。IPL-3-5和OPL-2的可视性因ART增加而改善。OSIZ-2和RPE-1、2、3、5在老年眼中的可视性比年轻眼中差。与健康老年眼相比，eAMD和iAMD中OSIZ-1-2、RPE-1和RPE-5的可视性降低。
我们使用一种用于HR-OCT的新型图像分析工具可靠地识别了28条视网膜条带。图像平均提高了内视网膜条带的可视性。衰老和AMD的发展影响了外视网膜条带的可视性。
对OCT上可见解剖结构的详细了解将提高研究的精度，包括人工智能训练和结构-功能分析。
