Aggarwal Dipanshu, Shetty Devi Charan, Jain Anshi, Gulati Nikita, Juneja Saurabh
Department of Oral Pathology and Microbiology, I.T.S-Centre for Dental Studies and Research, Muradnagar, Uttar Pradesh, India.
Department of Oral Pathology and Microbiology, Shree Bankey Bihari Dental College, Uttar Pradesh, India.
J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):287-294. doi: 10.4103/jomfp.jomfp_5_23. Epub 2023 Jul 13.
p53 tumour suppressor gene limits unchecked cellular growth in response to DNA damage, by causing G1 arrest and the activation of apoptosis. Inhibitors of apoptosis include survivin which acts by inhibition of caspases. Survivin has a significant role as a cell cycle modulator and is only minimally present in mature tissues. Aberrant expression of p53 and survivin has been evaluated in various carcinomas. Thus, the objective of this research was to elucidate the co-expression of p53 and survivin in tissue samples of Oral Potentially Malignant Disorders (OPMDs) and Oral Squamous Cell Carcinoma (OSCCs).
Thirty tissue samples of OPMDs and 30 tissue samples of OSCCs taken from department archives were used in the study. Expression of p53 and survivin was analyzed in the study groups by the help of immunohistochemistry. Also, co-expression of both the markers was evaluated.
The expression of p53 and survivin in the oral epithelium of patients with OSCCs was significantly higher than that in patients with OPMDs ( value ≤0.05).
Our results provide insights into the altered survivin and p53 co-expression with significant immunoexpression within the study groups. Therefore, survivin and p53 could be better markers for identifying cell proliferation and apoptotic pathway. Also, malignant transformation rate of OPMD increases with increased expression of these markers.
p53肿瘤抑制基因通过引起G1期阻滞和激活凋亡来限制DNA损伤时细胞的无节制生长。凋亡抑制因子包括通过抑制半胱天冬酶发挥作用的生存素。生存素作为一种细胞周期调节剂具有重要作用，且仅在成熟组织中微量存在。p53和生存素的异常表达已在多种癌症中得到评估。因此，本研究的目的是阐明口腔潜在恶性疾病（OPMDs）和口腔鳞状细胞癌（OSCCs）组织样本中p53和生存素的共表达情况。
本研究使用了从科室档案中获取的30份OPMDs组织样本和30份OSCCs组织样本。借助免疫组织化学分析研究组中p53和生存素的表达情况。此外，还评估了两种标志物的共表达情况。
OSCCs患者口腔上皮中p53和生存素的表达显著高于OPMDs患者（值≤0.05）。
我们的结果揭示了研究组中生存素和p53共表达改变以及显著的免疫表达情况。因此，生存素和p53可能是识别细胞增殖和凋亡途径的更好标志物。此外，OPMD的恶性转化率随这些标志物表达的增加而升高。
