Pradeepthi Katakam, Rajani Korlepara, Rao GuttikondaVenkateswara, Sravya Taneeru, Wahed Sarah Abdul, Sailaja Joparti
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):424. doi: 10.4103/jomfp.jomfp_146_22. Epub 2023 Jul 13.
Hematoxylin and eosin staining is the globally accepted staining technique for histology and histopathology sections. Hematoxylin is a natural dye as compared to eosin which is a synthetic dye manufactured from chemicals. Eosin acts as a counterstain to hematoxylin giving a sharp contrast to its blue color. Eosin being a chemical, can cause health hazards and environmental pollution. Using eco-friendly materials and going organic is the demand of this era. Curcuma longa rhizomes are proven to contain curcuminoids that exhibit various medicinal properties and curcumin the bright yellow component of the plant, that is, being used as a colorant. This natural dye from C. longa provides a significant alternative to eosin as they are safer to use without health hazards and are biodegradable and have easy disposability.
To evaluate and compare the staining qualities of C. longa (turmeric) with hematoxylin and eosin (H&E).
A total of 100 paraffin-embedded tissue block specimens of various pathological tissues were stained with H and E and hematoxylin and curcumin (H&C). Evaluation and comparison of the staining efficacy were done for all the pathological tissues. The obtained data were subjected to statistical analysis.
H&C staining gave comparable results with H and E staining with a statistically insignificant value ( > 0.05).
Curcumin has shown equivalent staining to that of eosin. Thus, it can be used as a better alternative.
苏木精和伊红染色是全球公认的用于组织学和组织病理学切片的染色技术。苏木精是一种天然染料，而伊红是一种由化学物质制成的合成染料。伊红作为苏木精的复染剂，与苏木精的蓝色形成鲜明对比。由于伊红是一种化学物质，会对健康造成危害并污染环境。使用环保材料和采用有机方法是这个时代的需求。姜黄根茎被证明含有具有多种药用特性的姜黄素类化合物，而姜黄素是这种植物的亮黄色成分，可作为着色剂。这种来自姜黄的天然染料是伊红的一种重要替代品，因为它们使用起来更安全，没有健康危害，可生物降解且易于处理。
评估并比较姜黄与苏木精和伊红（H&E）的染色质量。
用苏木精和伊红（H&E）以及苏木精和姜黄素（H&C）对总共100个不同病理组织的石蜡包埋组织块标本进行染色。对所有病理组织的染色效果进行评估和比较。对获得的数据进行统计分析。
H&C染色与H&E染色的结果相当，值无统计学意义（>0.05）。
姜黄素已显示出与伊红等效的染色效果。因此，它可以作为更好的替代品。
