Yin Fangjie, Chen Ying, Zhang Huijun, Zhao Hongzhe, Li Xuenan, Wang Zi, Meng Weijing, Zhao Jie, Tang Lijie, Li Yijing, Li Jiaxuan, Wang Xiaona
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, China.
Vet Sci. 2025 Apr 14;12(4):365. doi: 10.3390/vetsci12040365.
Trefoil factor 3 (TFF3) and epidermal growth factor (EGF) exert a promotive effect on the functions of intestinal epithelial cells and offer protection to the intestinal mucosa. can ameliorate intestinal mucosal damage. In this study, pPG-pTFF3/, pPG-pEGF/, and pPG-pTE/ were constructed to express porcine TFF3, EGF, and a fusion protein (pTE). Functional assays showed they promoted Immortalized Porcine Enterocyte Cell line J2 (IPEC-J2) proliferation and migration, with pTE having a greater migratory effect. In dextran sulfate sodium (DSS)-induced colitis mice, oral administration of pPG-pTE/ reduced colitis, improved mucosal integrity, increased the expression of tight-junction proteins and the serum level of Interleukin-10 (IL-10), and decreased the levels of pro-inflammatory Tumor Necrosis Factor-α (TNF-α), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β). These results imply that recombinant strains engineered to express pTFF3 and pEGF represent a promising approach for augmenting intestinal epithelial cell function and facilitating mucosal restitution, and they possess significant potential in the treatment of intestinal mucosal injury and inflammatory bowel disease (IBD).
三叶因子3（TFF3）和表皮生长因子（EGF）对肠上皮细胞功能具有促进作用，并为肠黏膜提供保护，可改善肠黏膜损伤。在本研究中，构建了pPG-pTFF3/、pPG-pEGF/和pPG-pTE/以表达猪TFF3、EGF和一种融合蛋白（pTE）。功能分析表明，它们促进了永生化猪肠上皮细胞系J2（IPEC-J2）的增殖和迁移，其中pTE具有更强的迁移作用。在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠中，口服pPG-pTE/可减轻结肠炎，改善黏膜完整性，增加紧密连接蛋白的表达以及白细胞介素-10（IL-10）的血清水平，并降低促炎细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的水平。这些结果表明，经基因工程改造以表达pTFF3和pEGF的重组菌株是增强肠上皮细胞功能和促进黏膜修复的一种有前景的方法，它们在治疗肠黏膜损伤和炎症性肠病（IBD）方面具有巨大潜力。
