Lobos E, Weiss N
Parasitology. 1986 Oct;93 ( Pt 2):389-99. doi: 10.1017/s0031182000051556.
Onchocerca volvulus proteins labelled with 125I were immunoprecipitated with onchocerciasis and lymphatic filariasis human serum pools in order to differentiate between cross-reacting and non-cross-reacting antigens. Analysis of the immunoprecipitates by two-dimensional gel electrophoresis revealed that all high molecular weight (Mr) O. volvulus antigens cross-reacted with the lymphatic filariasis serum pools. We observed, however, that at least 8 O. volvulus antigens were specifically immunoprecipitated only by the onchocerciasis serum pools, with Mr ranging from 20,000 up to 43,000 Daltons. These results suggest that the lower Mr O. volvulus antigens are more species specific than the other antigens. The significance of these findings for the immunodiagnosis of onchocerciasis is discussed.
用125I标记的盘尾丝虫蛋白与盘尾丝虫病和淋巴丝虫病患者血清库进行免疫沉淀反应，以区分交叉反应抗原和非交叉反应抗原。通过二维凝胶电泳对免疫沉淀物进行分析，结果显示所有高分子量盘尾丝虫抗原均与淋巴丝虫病血清库发生交叉反应。然而，我们观察到至少8种盘尾丝虫抗原仅能被盘尾丝虫病血清库特异性免疫沉淀，其分子量范围为20,000至43,000道尔顿。这些结果表明，较低分子量的盘尾丝虫抗原比其他抗原具有更高的种属特异性。文中讨论了这些发现对盘尾丝虫病免疫诊断的意义。
