利用患者血清和单克隆抗体对盘尾丝虫免疫显性抗原进行鉴定
Characterization of an immunodominant Onchocerca volvulus antigen with patient sera and a monoclonal antibody.
作者信息
Lucius R, Schulz-Key H, Büttner D W, Kern A, Kaltmann B, Prod'hon J, Seeber F, Walter R D, Saxena K C, Diesfeld H J
机构信息
Institut für Tropenhygiene, Universität Heidelberg, Federal Republic of Germany.
出版信息
J Exp Med. 1988 Apr 1;167(4):1505-10. doi: 10.1084/jem.167.4.1505.
Adult Onchocerca voluvlus and infective larvae, but not microfilariae contain an immunodominant antigen (33,000 and 21,000 Mr in females, 39,000, 33,000, and 21,000 Mr in males, 133,000 Mr in infective larvae) which is recognized by an Onchocerca-specific mAb. The component is part of the reproductive organs and muscles. 96.2% of onchocerciasis sera contained antibodies detectable by immunoblotting against it. Antigen purified by immunoaffinity chromatography was specifically recognized in immunoblots by onchocerciasis sera, but not by sera from other filarial infections. The high immunogenicity, the specificity, and the occurrence in infective larvae of this antigen indicate an immunodiagnostic potential and a possible role in the immunobiology of the parasite.
成虫盘尾丝虫和感染性幼虫而非微丝蚴含有一种免疫显性抗原（雌性中分子量为33,000和21,000，雄性中分子量为39,000、33,000和21,000，感染性幼虫中分子量为133,000），该抗原可被盘尾丝虫特异性单克隆抗体识别。该成分是生殖器官和肌肉的一部分。96.2%的盘尾丝虫病血清通过免疫印迹法可检测到针对它的抗体。通过免疫亲和层析纯化的抗原在免疫印迹中可被盘尾丝虫病血清特异性识别，但不能被其他丝虫感染的血清识别。该抗原的高免疫原性、特异性以及在感染性幼虫中的存在表明其具有免疫诊断潜力，并可能在寄生虫的免疫生物学中发挥作用。
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