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利用简化基因组测序进行全基因组关联研究，以鉴定甘薯抗镰刀菌根腐病的新位点和基因。

Genome-wide association study to identify novel loci and genes for Fusarium root rot resistance in sweet potato using genotyping-by-sequencing.

作者信息

Kim Tae Hwa, Kim Sujung, Park Won, Woo Koan Sik, Lee Keunpyo, Chung Mi Nam, Lee Young Hoon, Lee Hyeong-Un, Lee Kyo Hwui, Nam Sang-Sik, Jo Hyun, Lee Jeong-Dong

机构信息

Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea.

International Technology Cooperation Center, Technology Cooperation Bureau, Rural Development Administration, Jeonju, Republic of Korea.

出版信息

Front Plant Sci. 2023 Oct 4;14:1251157. doi: 10.3389/fpls.2023.1251157. eCollection 2023.

DOI:10.3389/fpls.2023.1251157
PMID:37860237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10584150/
Abstract

Fusarium root rot, caused by , is a major post-harvest disease in sweet potatoes ( (L.) Lam.). An effective strategy for controlling this disease is the development of resistant varieties. In this study, a genome-wide association study (GWAS) was conducted on 96 sweet potato genotypes to identify novel candidate loci and dissect the genetic basis of Fusarium root rot resistance. Genotyping was performed using genotyping-by-sequencing (GBS), and 44,255 SNPs were identified after filtering. The genotypes (n = 96) were evaluated through resistance tests in 2021 and 2022, separately and combined. The GWAS identified two significant SNP markers (LG3_22903756 and LG4_2449919) on chromosomes 3 and 4 associated with Fusarium root rot resistance, respectively. Lesion length showed significant differences between homozygous A and G alleles of LG3_22903756, which can potentially be used to develop molecular markers for selecting accessions resistant to Fusarium root rot. Expression analysis of 11 putative genes flanking the significant SNPs revealed the alteration in the expression of nine genes, indicating their possible involvement in Fusarium root rot resistance. The results of this study will aid in the marker-assisted selection and functional analysis of candidate genes for Fusarium root rot resistance in sweet potatoes.

摘要

由[病原菌名称未给出]引起的甘薯根腐病是甘薯（Ipomoea batatas (L.) Lam.）收获后的一种主要病害。控制这种病害的有效策略是培育抗病品种。在本研究中，对96个甘薯基因型进行了全基因组关联研究（GWAS），以鉴定新的候选基因座并剖析甘薯根腐病抗性的遗传基础。使用简化基因组测序（GBS）进行基因分型，过滤后鉴定出44,255个单核苷酸多态性（SNP）。在2021年和2022年分别及合并进行抗性测试，对96个基因型进行了评估。GWAS分别在3号和4号染色体上鉴定出两个与甘薯根腐病抗性相关的显著SNP标记（LG3_22903756和LG4_2449919）。LG3_22903756的纯合A和G等位基因之间的病斑长度显示出显著差异，这有可能用于开发分子标记来筛选抗甘薯根腐病的种质。对显著SNP侧翼的11个推定基因的表达分析表明，9个基因的表达发生了改变，表明它们可能参与了甘薯根腐病抗性。本研究结果将有助于甘薯根腐病抗性候选基因的标记辅助选择和功能分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/90ea1f24337d/fpls-14-1251157-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/9899ed39359d/fpls-14-1251157-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/2204da910b32/fpls-14-1251157-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/264368149106/fpls-14-1251157-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/fb28e812d788/fpls-14-1251157-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/6213d6ff10ec/fpls-14-1251157-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/d6ba009c6c46/fpls-14-1251157-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/01c44912e47f/fpls-14-1251157-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/90ea1f24337d/fpls-14-1251157-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/9899ed39359d/fpls-14-1251157-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/2204da910b32/fpls-14-1251157-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/264368149106/fpls-14-1251157-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/fb28e812d788/fpls-14-1251157-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/6213d6ff10ec/fpls-14-1251157-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/d6ba009c6c46/fpls-14-1251157-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/01c44912e47f/fpls-14-1251157-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b43/10584150/90ea1f24337d/fpls-14-1251157-g008.jpg

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本文引用的文献

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Transcriptome Characterization and Gene Changes Induced by in Sweetpotato Roots.转录组特征分析及 对甘薯根的基因变化诱导。
Genes (Basel). 2023 Apr 25;14(5):969. doi: 10.3390/genes14050969.
2
Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).遗传多样性评估和全基因组关联研究揭示了与甘薯（Ipomoea batatas (L.) Lam）组分性状相关的候选基因。
Mol Genet Genomics. 2023 May;298(3):653-667. doi: 10.1007/s00438-023-02007-3. Epub 2023 Mar 21.
3
Resequencing of sweetpotato germplasm resources reveals key loci associated with multiple agronomic traits.
甘薯种质资源的重测序揭示了与多个农艺性状相关的关键位点。
Hortic Res. 2022 Oct 19;10(1):uhac234. doi: 10.1093/hr/uhac234. eCollection 2023.
4
Natural allelic variation confers high resistance to sweet potato weevils in sweet potato.自然等位基因变异赋予甘薯对甘薯象鼻虫的高抗性。
Nat Plants. 2022 Nov;8(11):1233-1244. doi: 10.1038/s41477-022-01272-1. Epub 2022 Nov 14.
5
Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum.水杨酸通过抑制尖孢镰刀菌中雷帕霉素靶蛋白信号通路来防治枯萎病。
J Adv Res. 2022 Jul;39:1-13. doi: 10.1016/j.jare.2021.10.014. Epub 2021 Nov 2.
6
Exploring and exploiting genetics and genomics for sweetpotato improvement: Status and perspectives.探索和利用遗传学和基因组学改良甘薯：现状与展望。
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Incidence Rates of Root Rot in Sweetpotato Caused by Cultivation Soil and Soil Microorganisms During Storage Periods.贮藏期栽培土壤和土壤微生物引起的甘薯根腐病发病率
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