University Institute of Medical Laboratory Technology, The University of Lahore, 54000, Pakistan.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Curr Stem Cell Res Ther. 2024;19(9):1286-1292. doi: 10.2174/011574888X260118230927050143.
Umbilical cord mesenchymal stem cells (UC-MSCs) are increasingly being utilized for immune-related disease therapies due to their low immunogenicity. However, the primary culture of UC-MSCs requires the supplementation of serum in the growth medium, which has posed a challenge due to ethical issues related to the collection method of the fetal bovine serum (FBS) that is routinely used in cell culture.
In order to address this, the purpose of this research was to assess the effectiveness of adult bovine serum (ABS) as a different and more affordable source of serum for the in-vitro cultivation of UC-MSCs. UC-MSCs were isolated from the umbilical cord of Wharton's jelly of cow immediately after birth, by digestion with Collagenase type I.
ABS was collected from fresh bovine sources and heat-inactivated. The morphology of UC-MSCs was observed under an inverted microscope, and growth patterns, proliferative index, and doubling time were calculated every two days to compare the efficacy of ABS with FBS. Immunocytochemistry for specific markers was also conducted on the MSCs.
The results showed a notable difference in morphology, growth rate, population doubling, and proliferative index between ABS and FBS.
Intriguingly, ABS proved to be an effective supplement in the growth medium for expanding UC-MSCs , providing a viable alternative to FBS.
由于脐带间充质干细胞 (UC-MSCs) 的免疫原性较低，因此越来越多地被用于免疫相关疾病的治疗。然而，UC-MSCs 的原代培养需要在生长培养基中补充血清，由于常规用于细胞培养的胎牛血清 (FBS) 的采集方法存在伦理问题，这给培养带来了挑战。
为了解决这个问题，本研究旨在评估成牛血清 (ABS) 作为 UC-MSCs 体外培养的不同且更经济实惠的血清来源的有效性。UC-MSCs 是从牛的脐带华通氏胶中立即分离出来的，通过胶原酶 I 消化。
从新鲜的牛源中收集 ABS 并进行热灭活。倒置显微镜下观察 UC-MSCs 的形态，每隔两天计算生长模式、增殖指数和倍增时间，以比较 ABS 与 FBS 的效果。还对 MSCs 进行了特定标志物的免疫细胞化学染色。
结果显示 ABS 与 FBS 在形态、生长速度、群体倍增和增殖指数方面存在显著差异。
有趣的是，ABS 被证明是 UC-MSCs 生长培养基中的有效补充物，为 FBS 提供了可行的替代物。
