Institute of Hydrobiology, College of Modern Agriculture Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences , Wuhan, China.
The Innovation Academy of Seed Design, Chinese Academy of Sciences , Beijing, China.
J Virol. 2023 Nov 30;97(11):e0122623. doi: 10.1128/jvi.01226-23. Epub 2023 Oct 20.
Although a virus can regulate many cellular responses to facilitate its replication by interacting with host proteins, the host can also restrict virus infection through these interactions. In the present study, we showed that the host eukaryotic translation elongation factor 1 alpha (eEF1A), an essential protein in the translation machinery, interacted with two proteins of a fish rhabdovirus, rhabdovirus (SCRV), and inhibited virus infection via two different mechanisms: (i) inhibiting the formation of crucial viral protein complexes required for virus transcription and replication and (ii) promoting the ubiquitin-proteasome degradation of viral protein. We also revealed the functional regions of eEF1A that are involved in the two processes. Such a host protein inhibiting a rhabdovirus infection in two ways is rarely reported. These findings provided new information for the interactions between host and fish rhabdovirus.
虽然病毒可以通过与宿主蛋白相互作用来调节许多细胞反应，从而促进其复制，但宿主也可以通过这些相互作用来限制病毒感染。在本研究中，我们表明，宿主真核翻译延伸因子 1 阿尔法（eEF1A）是翻译机制中的一种必需蛋白，与两种鱼类弹状病毒（SCRV）的蛋白相互作用，并通过两种不同的机制抑制病毒感染：（i）抑制形成病毒转录和复制所必需的关键病毒蛋白复合物；（ii）促进病毒蛋白的泛素-蛋白酶体降解。我们还揭示了 eEF1A 参与这两个过程的功能区域。这种以两种方式抑制弹状病毒感染的宿主蛋白很少有报道。这些发现为宿主与鱼类弹状病毒之间的相互作用提供了新的信息。
