Niu Yinjie, Yang Xinmei, Liang Hongru, Luo Xia, Ma Baofu, Lin Qiang, Fu Xiaozhe, Li Ningqiu
Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Key Laboratory of Fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Viruses. 2024 Dec 2;16(12):1876. doi: 10.3390/v16121876.
Tripartite Motif-Containing 44 (TRIM44) is responsible for cancers, neurodegenerative diseases, and viral infections. However, the role of TRIM44 (scTRIM44) during viral infection remains unclear. In the present study, we analyzed the molecular characteristics of scTRIM44 and its role in infectious spleen and kidney necrosis virus (ISKNV), largemouth bass virus (LMBV), and Siniperca chuatsi rhabdovirus (SCRV) infection. ScTRIM44 contained one B-box domain (B, 166-207 aa) and a coiled-coil domain (CC, 279-309 aa), but lacked the canonical RING domain of E3 ubiquitin ligases. The scTRIM44 mRNA was expressed relatively high in immune-related tissues. The mRNA expression of scTRIM44 significantly decreased in vivo and vitro post-ISKNV and -LMBV infection. However, the expression of scTRIM44 mRNA showed significant up-regulation post-SCRV infection. ScTRIM44 positively regulated SCRV infection in CPB cells, but copies of ISKNV and LMBV showed no significant alteration in over-expressed or knocked-down scTRIM44 cells. Moreover, scTRIM44 positively regulated RIG-I- and MDA5-mediated interferon molecule signaling. These data suggested that scTRIM44 promoted SCRV infection by positively regulating RIG-I- and MDA5-mediated interferon molecule signaling, but didn't regulate ISKNV and LMBV infection. This research provided a comprehensive insight into the antiviral activity of scTRIM44.
含三联基序蛋白44（TRIM44）与癌症、神经退行性疾病和病毒感染有关。然而，TRIM44（硬骨鱼TRIM44，scTRIM44）在病毒感染过程中的作用仍不清楚。在本研究中，我们分析了scTRIM44的分子特征及其在传染性脾肾坏死病毒（ISKNV）、大口黑鲈病毒（LMBV）和鳜鱼弹状病毒（SCRV）感染中的作用。scTRIM44包含一个B-盒结构域（B，第166 - 207位氨基酸）和一个卷曲螺旋结构域（CC，第279 - 309位氨基酸），但缺乏E3泛素连接酶的典型RING结构域。scTRIM44 mRNA在免疫相关组织中表达相对较高。在ISKNV和LMBV感染后，scTRIM44的mRNA表达在体内和体外均显著下降。然而，在SCRV感染后，scTRIM44 mRNA的表达显著上调。scTRIM44在鲤上皮瘤细胞（CPB细胞）中正向调节SCRV感染，但在过表达或敲低scTRIM44的细胞中，ISKNV和LMBV的拷贝数没有显著变化。此外，scTRIM44正向调节视黄酸诱导基因I（RIG-I）和黑色素瘤分化相关基因5（MDA5）介导的干扰素分子信号传导。这些数据表明，scTRIM44通过正向调节RIG-I和MDA5介导的干扰素分子信号传导促进SCRV感染，但不调节ISKNV和LMBV感染。本研究为scTRIM44的抗病毒活性提供了全面的见解。
