Wu Yuehong, Ren Wen-Ting, Zhong Ying-Wen, Guo Li-Li, Zhou Peng, Xu Xue-Wei
Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
School of Oceanography, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005286.
Two strains (GL-11-2 and ZH2-Y79) were isolated from the seawater collected from the West Pacific Ocean and the East China Sea, respectively. Cells were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Cells grew in the medium containing 0.5-7.5 % NaCl (w/v, optimum, 1.0-3.0 %), at pH 6.0-8.0 (optimum, pH 6.5-7.0) and at 4-40 °C (optimum, 30 °C). HS production occurred in marine broth supplemented with sodium thiosulphate. The almost-complete 16S rRNA gene sequences of the two isolates were identical, and exhibited the highest similarity to JCM 13603 (97.5 %), followed by TW15 (97.2%), DSM 15283 (97.1 %) and ITI-1157 (97.0 %). Phylogenetic analysis revealed that the isolates were affiliated with the family and represented an independent lineage. The sole isoprenoid quinone was ubiquinone 10. The principal fatty acids were summed feature 8 (C 7 and/or C 6) and cyclo-C 8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol. The DNA G+C content was 62.3 mol%. The orthologous average nucleotide identity, DNA-DNA hybridization and average amino acid identity values among the genomes of strain GL-11-2 and the reference strains were 73.2-79.0, 20.3-22.5 and 66.0-80.8 %, respectively. Strains GL-11-2ᵀ and ZH2-Y79 possessed complete metabolic pathways for thiosulphate oxidation, dissimilatory nitrate reduction and denitrification. Phylogenetic distinctiveness, chemotaxonomic differences and phenotypic properties revealed that the isolates represent a novel genus and species of the family , belonging to the class , for which the name gen. nov., sp. nov. (type strain, GL-11-2=KCTC 82723=MCCC M20691) is proposed.
分别从西太平洋和东海采集的海水中分离出两株菌株（GL-11-2和ZH2-Y79）。细胞革兰氏染色阴性，严格需氧，无运动性，呈杆状。细胞在含0.5-7.5%（w/v，最适为1.0-3.0%）NaCl、pH 6.0-8.0（最适pH 6.5-7.0）和4-40℃（最适30℃）的培养基中生长。在补充有硫代硫酸钠的海洋肉汤中产生硫化氢。两株分离株的近乎完整的16S rRNA基因序列相同，与JCM 13603的相似性最高（97.5%），其次是TW15（97.2%）、DSM 15283（97.1%）和ITI-1157（97.0%）。系统发育分析表明，这些分离株属于该科，代表一个独立的谱系。唯一的类异戊二烯醌是泛醌10。主要脂肪酸是总和特征8（C17:1ω6c和/或C16:1ω7c）和环C18:1ω7c。主要极性脂是磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和二磷脂酰甘油。DNA G+C含量为62.3mol%。菌株GL-11-2与参考菌株基因组之间的直系同源平均核苷酸同一性、DNA-DNA杂交和平均氨基酸同一性值分别为73.2-79.0、20.3-22.5和66.0-80.8%。菌株GL-11-2T和ZH2-Y79具有硫代硫酸盐氧化、异化硝酸盐还原和反硝化的完整代谢途径。系统发育独特性、化学分类学差异和表型特性表明，这些分离株代表该科的一个新属和新种，属于该纲，为此提出新属名，新种名（模式菌株，GL-11-2=KCTC 82723=MCCC M20691）。
