阳离子卟啉作为DNA结构的探针
Cationic porphyrins as probes of DNA structure.
作者信息
Bromley S D, Ward B W, Dabrowiak J C
出版信息
Nucleic Acids Res. 1986 Nov 25;14(22):9133-48. doi: 10.1093/nar/14.22.9133.
Abstract
The DNA binding specificity of a group of cationic manganese porphyrin complexes has been examined using DNase I footprinting methodology and by observing the sites of porphyrin-induced DNA strand scission in the presence of potassium superoxide. The compounds, which possess systematic changes in total charge, its distribution on the periphery on the macrocycle and ligand shape, bind in the minor groove of AT rich regions of DNA. While changes in total charge and charge arrangement do not significantly influence specificity, a shape change which blocks close ligand contact with the minor groove relaxes the original AT specificity causing the compound to cleave at both AT and GC sites. The observed changes in binding sequence specificity were interpreted in terms of electrostatic and steric factors associated with both the compounds and DNA.
摘要
使用DNA酶I足迹法并通过观察在超氧化钾存在下卟啉诱导的DNA链断裂位点，研究了一组阳离子锰卟啉配合物的DNA结合特异性。这些化合物在总电荷、其在大环外围的分布以及配体形状方面具有系统性变化，它们结合在DNA富含AT区域的小沟中。虽然总电荷和电荷排列的变化不会显著影响特异性，但一种阻止配体与小沟紧密接触的形状变化会放松原来的AT特异性，导致该化合物在AT和GC位点均发生切割。根据与化合物和DNA相关的静电和空间因素，对观察到的结合序列特异性变化进行了解释。
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