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通过足迹法揭示的一种嵌入卟啉化合物的DNA序列偏好性。

DNA sequence preferences for an intercalating porphyrin compound revealed by footprinting.

作者信息

Ford K, Fox K R, Neidle S, Waring M J

出版信息

Nucleic Acids Res. 1987 Mar 11;15(5):2221-34. doi: 10.1093/nar/15.5.2221.

DOI:10.1093/nar/15.5.2221
PMID:3562226
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340629/
Abstract

The DNA sequence preferences of the compound meso-tetra-(4-N-methyl(pyridyl) porphyrin and its nickel complex have been investigated by means of footprinting experiments on several DNA fragments, using DNAase I and micrococcal nuclease as footprinting agents. A complex pattern of both AT and GC-protected sites was found. Ligand-induced long-range conformational changes were inferred in several instances to be related to the observed large-scale blockages of enzymatic cutting.

摘要

通过使用脱氧核糖核酸酶I和微球菌核酸酶作为足迹试剂，对几个DNA片段进行足迹实验，研究了化合物中-四-（4-N-甲基（吡啶基）卟啉及其镍配合物的DNA序列偏好。发现了由AT和GC保护位点组成的复杂模式。在几种情况下，推断配体诱导的长程构象变化与观察到的酶切大规模阻断有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/15a1e92911a2/nar00249-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/e23dc2cde3c5/nar00249-0341-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/6bd763cc6d92/nar00249-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/24e413e32a7a/nar00249-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/15a1e92911a2/nar00249-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/e23dc2cde3c5/nar00249-0341-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/6bd763cc6d92/nar00249-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/24e413e32a7a/nar00249-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/340629/15a1e92911a2/nar00249-0347-a.jpg

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本文引用的文献

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Sequence specific cleavage of DNA by micrococcal nuclease.微球菌核酸酶对DNA的序列特异性切割。
Nucleic Acids Res. 1981 Jun 25;9(12):2643-58. doi: 10.1093/nar/9.12.2643.
2
Binding of meso-tetra (4-N-methylpyridyl) porphine to DNA.中位-四（4-N-甲基吡啶基）卟啉与DNA的结合
Nucleic Acids Res. 1980 Jun 25;8(12):2835-42. doi: 10.1093/nar/8.12.2835.
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Intercalative and nonintercalative binding of large cationic porphyrin ligands to polynucleotides.大阳离子卟啉配体与多核苷酸的插入和非插入结合
Sequence-dependent reactivity of linear DNA to chemical cleavage by Cu(II):thiol combinations including cysteine or glutathione.
线性DNA对Cu(II)与包括半胱氨酸或谷胱甘肽在内的硫醇组合进行化学切割的序列依赖性反应活性。
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Molecular modelling of the interactions of tetra-(4-N-methylpyridyl) porphin with TA and CG sites on DNA.四（4-N-甲基吡啶基）卟啉与DNA上TA和CG位点相互作用的分子模拟
Nucleic Acids Res. 1987 Aug 25;15(16):6553-62. doi: 10.1093/nar/15.16.6553.
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A molecular model for proflavine-DNA intercalation.原黄素-DNA嵌入的分子模型。
Nucleic Acids Res. 1988 Sep 26;16(18):8999-9016. doi: 10.1093/nar/16.18.8999.
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Interactions of water-soluble metalloporphyrins with nucleic acids studied by resonance Raman spectroscopy.通过共振拉曼光谱研究水溶性金属卟啉与核酸的相互作用。
Nucleic Acids Res. 1988 Nov 11;16(21):10323-38. doi: 10.1093/nar/16.21.10323.
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Modelling of the binding specificity in the interactions of cationic porphyrins with DNA.阳离子卟啉与DNA相互作用中结合特异性的建模
Nucleic Acids Res. 1990 Mar 11;18(5):1109-14. doi: 10.1093/nar/18.5.1109.
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Interactions of porphyrins with nucleic acids.卟啉与核酸的相互作用。
Biochemistry. 1983 May 10;22(10):2406-14. doi: 10.1021/bi00279a016.
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31P NMR and viscometric studies of the interaction of meso-tetra(4-N-methylpyridyl) porphine and its Ni(II) and Zn(II) derivatives with DNA.31P核磁共振及黏度测定法研究中位-四(4-N-甲基吡啶基)卟啉及其镍(II)和锌(II)衍生物与DNA的相互作用
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Interactions of porphyrins with nucleic acids.卟啉与核酸的相互作用。
Biochemistry. 1983 Nov 8;22(23):5409-17. doi: 10.1021/bi00292a024.
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Molecular models for the interaction of the anti-tumour drug nogalamycin with DNA.抗肿瘤药物诺加霉素与DNA相互作用的分子模型
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DNA structural variations in the E. coli tyrT promoter.大肠杆菌tyrT启动子中的DNA结构变异
Cell. 1984 Jun;37(2):491-502. doi: 10.1016/0092-8674(84)90379-9.
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High sequence specificity of micrococcal nuclease.微球菌核酸酶的高序列特异性。
Nucleic Acids Res. 1981 Jun 25;9(12):2659-73. doi: 10.1093/nar/9.12.2659.