通过足迹法揭示的一种嵌入卟啉化合物的DNA序列偏好性。

Ford K, Fox K R, Neidle S, Waring M J

Nucleic Acids Res. 1987 Mar 11;15(5):2221-34. doi: 10.1093/nar/15.5.2221.

The DNA sequence preferences of the compound meso-tetra-(4-N-methyl(pyridyl) porphyrin and its nickel complex have been investigated by means of footprinting experiments on several DNA fragments, using DNAase I and micrococcal nuclease as footprinting agents. A complex pattern of both AT and GC-protected sites was found. Ligand-induced long-range conformational changes were inferred in several instances to be related to the observed large-scale blockages of enzymatic cutting.

通过使用脱氧核糖核酸酶I和微球菌核酸酶作为足迹试剂，对几个DNA片段进行足迹实验，研究了化合物中-四-（4-N-甲基（吡啶基）卟啉及其镍配合物的DNA序列偏好。发现了由AT和GC保护位点组成的复杂模式。在几种情况下，推断配体诱导的长程构象变化与观察到的酶切大规模阻断有关。